Effectiveness of infection-containment measures on SARS-CoV-2 seroprevalence and circulation from May to July 2020, in Milan, Italy.
Effectiveness of infection-containment measures on SARS-CoV-2 seroprevalence and circulation from May to July 2020, in Milan, Italy.
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DOI：
10.1371/journal.pone.0242765
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Perno CF
中科院分区：
文献类型：
--
作者：
Cento V;Alteri C;Merli M;Di Ruscio F;Tartaglione L;Rossotti R;Travi G;Vecchi M;Raimondi A;Nava A;Colagrossi L;Fumagalli R;Ughi N;Epis OM;Fanti D;Beretta A;Galbiati F;Scaglione F;Vismara C;Puoti M;Campisi D;Perno CF
通过基于医院的SARS-CoV-2分子和血清学筛查，我们评估了伦巴第米兰两个月封锁和两个月监测的有效性，米兰是2020年3月至4月期间第一个被COVID-19大流行淹没的城市。2020年5月11日至7月5日在米兰主要医院就诊的所有受试者均通过荧光测定法进行了血清学筛查。这些被录取的人进一步进行了RT-PCR检测。在分析的2753例受试者中，累积抗-N IgG血清阳性率为5.1%（95%CI = 4.3%-6.0%），自诊断高峰（3月-20日）起60-63天达到峰值8.4%（6.1%-11.4%）。31/106例（29.2%）抗N反应受试者的抗S1/S2滴度>80 Au/mL。在2018年5月至2019年6月5日期间进行检测或居住在SARS-CoV-2流行较高的省份与抗-N IgG反应性呈独立正相关（OR [95%CI]分别为：2.179[1.455-3.264]和3.127[1.18-8.29]）。在18例RT-PCR阳性、有症状的受试者中，抗N血清阳性率为33.3%（95%CI：14.8%-56.3%）。米兰的SARS-CoV-2血清阳性率较低，并且在诊断高峰后仅60-63天后呈下降趋势。意大利的限制措施是有效的，但蔓延的风险仍然是具体的。在医院环境中，入院时进行分子和血清学筛查仍然是非常可取的。
Through a hospital-based SARS-CoV-2 molecular and serological screening, we evaluated the effectiveness of two months of lockdown and two of surveillance, in Milan, Lombardy, the first to be overwhelmed by COVID-19 pandemics during March-April 2020. All subjects presenting at the major hospital of Milan from May-11 to July-5, 2020, underwent a serological screening by chemiluminescent assays. Those admitted were further tested by RT-PCR. The cumulative anti-N IgG seroprevalence in the 2753 subjects analyzed was of 5.1% (95%CI = 4.3%-6.0%), with a peak of 8.4% (6.1%-11.4%) 60–63 days since the peak of diagnoses (March-20). 31/106 (29.2%) anti-N reactive subjects had anti-S1/S2 titers >80 AU/mL. Being tested from May-18 to June-5, or residing in the provinces with higher SARS-CoV-2 circulation, were positively and independently associated with anti-N IgG reactivity (OR [95%CI]: 2.179[1.455–3.264] and 3.127[1.18–8.29], respectively). In the 18 RT-PCR positive, symptomatic subjects, anti-N seroprevalence was 33.3% (95% CI: 14.8%-56.3%). SARS-CoV-2 seroprevalence in Milan is low, and in a downward trend after only 60–63 days since the peak of diagnoses. Italian confinement measures were effective, but the risk of contagion remains concrete. In hospital-settings, the performance of molecular and serological screenings upon admission remains highly advisable.
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