Serological assays for emerging coronaviruses: challenges and pitfalls.
Serological assays for emerging coronaviruses: challenges and pitfalls.
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DOI：
10.1016/j.virusres.2014.03.018
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发表时间：
2014-12-19
期刊：
影响因子：
5
通讯作者：
Müller MA
中科院分区：
文献类型：
--
作者：
Meyer B;Drosten C;Müller MA
综述了SARS和MERS冠状病毒（CoV）诊断的血清学研究。比较了不同类型的血清学检测方法和可变抗原。冠状病毒刺突蛋白的免疫原性表位比核衣壳蛋白保守性低。发现刺突蛋白的使用上级核衣壳蛋白。综述了血清学方法在动物血清分析中的应用。在2002/2003年严重急性呼吸综合征冠状病毒（SARS-CoV）出现十多年后，一种名为中东呼吸综合征（MERS）CoV的新型CoV的出现挑战了研究人员和公共卫生当局。为了控制传播并最终遏制新病毒，快速识别并随后隔离感染者及其接触者至关重要。除了核酸检测方法之外，经验证的血清学测定尤其重要，因为抗体检测的时间范围限制较少。在SARS-CoV流行期间，使用多种方法以及不同的病毒抗原建立了多种血清学诊断测定。尽管大多数开发的检测方法显示出高灵敏度和特异性，但许多研究报告了对来自广泛传播的普通感冒相关CoV的抗原的交叉反应性抗体。为了提高对未来新型冠状病毒爆发的准备和反应能力，应了解SARS-CoV期间发生的血清学诊断信息和问题。在这篇综述中，我们总结了不同血清学检测的性能以及SARS-CoV爆发期间使用的两种主要应用抗原（刺突和核壳蛋白）的适用性。我们强调了在处理MERS-CoV等新型冠状病毒时出现的挑战和潜在陷阱。此外，我们描述了可能发生的问题，当动物血清进行血清学试验，以确定推定水库。最后，我们给出了一个血清学测试计划的建议，并概述了必要的改进，应实施更好的准备。
Serological studies on SARS- and MERS-coronavirus (CoV) diagnostics were reviewed. Different types of serological assays and variable antigens were compared. Immunogenic epitopes of CoV spike proteins were less conserved than nucleocapsid proteins. Use of spike proteins was found to be superior over nucleocapsid proteins. Applicability of serological assays for analysis of animal sera was reviewed. More than a decade after the emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002/2003 the occurrence of a novel CoV termed Middle East respiratory syndrome (MERS) CoV challenges researchers and public health authorities. To control spread and finally contain novel viruses, rapid identification and subsequent isolation of infected individuals and their contacts is of utmost importance. Next to methods for nucleic acid detection, validated serological assays are particularly important as the timeframe for antibody detection is less restricted. During the SARS-CoV epidemic a wide variety of serological diagnostic assays were established using multiple methods as well as different viral antigens. Even though the majority of the developed assays showed high sensitivity and specificity, numerous studies reported on cross-reactive antibodies to antigens from wide-spread common cold associated CoVs. In order to improve preparedness and responsiveness during future outbreaks of novel CoVs, information and problems regarding serological diagnosis that occurred during the SARS-CoV should be acknowledged. In this review we summarize the performance of different serological assays as well as the applicability of the two main applied antigens (spike and nucleocapsid protein) used during the SARS-CoV outbreak. We highlight challenges and potential pitfalls that occur when dealing with a novel emerging coronavirus like MERS-CoV. In addition we describe problems that might occur when animal sera are tested in serological assays for the identification of putative reservoirs. Finally, we give a recommendation for a serological testing scheme and outline necessary improvements that should be implemented for a better preparedness.
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DOI：
10.1007/978-1-59745-181-9_23
发表时间：
2008
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Fukushi S;Watanabe R;Taguchi F
通讯作者：
Taguchi F
影响因子：
11.8
作者：
Chan PK;Ng KC;Chan RC;Lam RK;Chow VC;Hui M;Wu A;Lee N;Yap FH;Cheng FW;Sung JJ;Tam JS
通讯作者：
Tam JS
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
8.8
作者：
Chan, C. M.;Tse, Herman;Yuen, K. Y.
通讯作者：
Yuen, K. Y.
影响因子：
1.5
作者：
Ecker, B;Vollenhofer, S;Pitner, F
通讯作者：
Pitner, F