Resequencing of Capsicum annuum parental lines (YCM334 and Taean) for the genetic analysis of bacterial wilt resistance.
Resequencing of Capsicum annuum parental lines (YCM334 and Taean) for the genetic analysis of bacterial wilt resistance.
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DOI：
10.1186/s12870-016-0931-0
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发表时间：
2016-10-28
影响因子：
5.3
通讯作者：
Jun TH
中科院分区：
文献类型：
--
作者：
Kang YJ;Ahn YK;Kim KT;Jun TH
细菌性枯萎病（BW）是一种广泛存在的植物病害，影响广泛的双子叶和单子叶寄主，对茄类植物尤其有害，如辣椒、番茄和茄子。导致BW的病原体是土壤传播的细菌，Ralstonia solanacearum，它可以适应不同的温度条件，在热带到温带的气候中都有发现。部分辣椒株系已检测到对BW的抗性；然而，介导这种情况的基因组位点和等位基因在该物种中研究甚少。我们对辣椒品种YCM344和Taean进行了重测序，这两个亲本重组自交系（RIL）对BW表现出不同的抗性表型，YCM344对该病菌的感染具有高度抗性。与参考基因组相比，我们发现了仅存在于两个亲本系中的新的单核苷酸多态性（SNPs）和插入/缺失（Indels），并进一步确定了区分这两个品种的变异。然后，我们在先前与抗病相关的基因（如R基因和应激反应基因）中发现了具有潜在信息的snp。此外，通过比较分析，我们确定了位于基因组区域的snp与番茄基因组中已知的抗性基因具有同源性。从我们对两个亲本系的SNP分析中，我们可以确定可能导致BW抗性的SNP，并且实际上，这些SNP可以用作使用这些群体的辅助育种计划的标记。我们预测，我们的分析将有助于更好地了解YCM334/ taean衍生群体，以及增强我们对辣椒基因组中存在的关键snp的了解。本文的在线版本（doi:10.1186/s12870-016-0931-0）包含补充材料，可供授权用户使用。
Bacterial wilt (BW) is a widespread plant disease that affects a broad range of dicot and monocot hosts and is particularly harmful for solanaceous plants, such as pepper, tomato, and eggplant. The pathogen responsible for BW is the soil-borne bacterium, Ralstonia solanacearum, which can adapt to diverse temperature conditions and is found in climates ranging from tropical to temperate. Resistance to BW has been detected in some pepper plant lines; however, the genomic loci and alleles that mediate this are poorly studied in this species. We resequenced the pepper cultivars YCM344 and Taean, which are parental recombinant inbred lines (RIL) that display differential resistance phenotypes against BW, with YCM344 being highly resistant to infection with this pathogen. We identified novel single nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) that are only present in both parental lines, as compared to the reference genome and further determined variations that distinguish these two cultivars from one another. We then identified potentially informative SNPs that were found in genes related to those that have been previously associated with disease resistance, such as the R genes and stress response genes. Moreover, via comparative analysis, we identified SNPs located in genomic regions that have homology to known resistance genes in the tomato genomes. From our SNP profiling in both parental lines, we could identify SNPs that are potentially responsible for BW resistance, and practically, these may be used as markers for assisted breeding schemes using these populations. We predict that our analyses will be valuable for both better understanding the YCM334/Taean-derived populations, as well as for enhancing our knowledge of critical SNPs present in the pepper genome. The online version of this article (doi:10.1186/s12870-016-0931-0) contains supplementary material, which is available to authorized users.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
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DOI：
10.1073/pnas.1400975111
发表时间：
2014-04-08
影响因子：
11.1
作者：
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通讯作者：
Zhang, Zhiming
影响因子：
2.7
作者：
Kim, Bong-Suk;French, Elizabeth;Iyer-Pascuzzi, Anjali S.
通讯作者：
Iyer-Pascuzzi, Anjali S.
影响因子：
4.5
作者：
Ji, Pingsheng;Allen, Caitilyn;Momol, A. Timur
通讯作者：
Momol, A. Timur
影响因子：
13.8
作者：
Kang YJ;Lee T;Lee J;Shim S;Jeong H;Satyawan D;Kim MY;Lee SH
通讯作者：
Lee SH