Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1
Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1
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酿酒酵母减数分裂的诱导取决于转录抑制子 Ume6 通过与转录激活子 Ime1 的调节关联而转变为正调节子
DOI：
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发表时间：
1996
影响因子：
5.3
通讯作者：
Y. Kassir
中科院分区：
文献类型：
--
作者：
I. Rubin;S. Mandel;K. Robzyk;Y. Kassir
减数分裂特异性基因的转录，以及减数分裂的启动，在芽殖酵母酿酒酵母依赖于IME1。IME1编码缺乏已知DNA结合基序的转录激活因子。在这项研究中，我们已经确定的模式，Ime1特异性激活减数分裂基因的转录。我们证明，Ime1被招募到减数分裂基因的启动子与DNA结合蛋白，Ume6相互作用。Ime1和Ume6之间的这种关联依赖于饥饿和由RIM 11编码的蛋白激酶的活性。在Ime1缺失的情况下，Ume6抑制减数分裂基因的转录。然而，在存在Ime1的情况下，或者当Ume6与Gal4激活结构域框内融合时，Ume6从阻遏物转化为激活物，导致减数分裂特异性基因的转录和子囊的形成。
The transcription of meiosis-specific genes, as well as the initiation of meiosis, in the budding yeast Saccharomyces cerevisiae depends on IME1. IME1 encodes a transcriptional activator which lacks known DNA binding motifs. In this study we have determined the mode by which Ime1 specifically activates the transcription of meiotic genes. We demonstrate that Ime1 is recruited to the promoters of meiotic genes by interacting with a DNA-binding protein, Ume6. This association between Ime1 and Ume6 depends on both starvation and the activity of a protein kinase, encoded by RIM11 In the absence of Ime1, Ume6 represses the transcription of meiotic genes. However, in the presence of Ime1, or when Ume6 is fused in frame to the Gal4 activation domain, Ume6 is converted from a repressor to an activator, resulting in the transcription of meiosis-specific genes and the formation of asci.
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影响因子：
10.5
作者：
DURFEE, T;BECHERER, K;ELLEDGE, SJ
通讯作者：
ELLEDGE, SJ
用于构建trpE融合基因的具有多个克隆位点的高表达载体：pATH载体。
影响因子：
--
作者：
Koerner,TJ;Hill,JE;Myers,AM;Tzagoloff,A
通讯作者：
Tzagoloff,A
酵母 IME1 基因产物转录激活的遗传证据。
影响因子：
3.3
作者：
Smith,HE;Driscoll,SE;Sia,RA;Yuan,HE;Mitchell,AP
通讯作者：
Mitchell,AP
DOI：
10.1073/pnas.88.21.9578
发表时间：
1991-11-01
影响因子：
11.1
作者：
CHIEN, CT;BARTEL, PL;FIELDS, S
通讯作者：
FIELDS, S
影响因子：
--
作者：
M. Rose;D. Botstein
通讯作者：
M. Rose;D. Botstein