Isolation of Staphylococcus aureus and antibiotic-resistant Staphylococcus aureus from residential indoor bioaerosols.
Isolation of Staphylococcus aureus and antibiotic-resistant Staphylococcus aureus from residential indoor bioaerosols.
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DOI：
10.1289/ehp.9585
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发表时间：
2006-12
影响因子：
10.4
通讯作者：
Gibbs SG
中科院分区：
文献类型：
--
作者：
Gandara A;Mota LC;Flores C;Perez HR;Green CF;Gibbs SG
在这项研究中，我们评估了金黄色葡萄球菌和耐药链球菌的水平。金黄色葡萄球菌的菌落形成单位（CFU）/立方米空气。我们使用Andersen两阶段采样器从德克萨斯州埃尔帕索市的24个家庭采集生物气溶胶样品，以胰蛋白酶大豆琼脂为收集培养基，然后在Chapman Stone选择性培养基上重复平板法分离S.金黄色。Kirby-Bauer纸片扩散法用于测定对氨苄西林、青霉素和头孢克洛的抗生素耐药性，这三种抗生素代表两种不同的抗生素类别。每户室外可吸入异养菌平均回收浓度为345.38 CFU/m3，其中沙门氏菌平均为12.63 CFU/m3。金黄色。平均回收浓度为460.23 CFU/m3，其中S.金黄色。呼吸性S.从每户家庭内回收的金黄色葡萄球菌对氨比西林的平均耐药率为54.59%，对氨比西林的平均耐药率为60.46%。青霉素。存在头孢克洛耐药和多重耐药的S。金黄色葡萄球菌感染率为13.20%。呼吸性S.平均耐药率为34.42%，青霉素为41.81%。存在头孢克洛耐药和多重耐药的S。金黄色葡萄球菌感染率为13.96%。这项研究表明，抗疟疾的生物气溶胶通常在居民家中发现。我们的研究结果还表明，耐药菌株的空气可培养的S。金黄色葡萄球菌在研究家庭内的浓度高于家庭外。
In this study we evaluated the levels of Staphylococcus aureus and antibiotic-resistant S. aureus in colony-forming units (CFU) per cubic meter of air. We used Andersen two-stage samplers to collect bioaerosol samples from 24 houses in El Paso, Texas, using tryptic soy agar as the collection media, followed by the replicate plate method on Chapman Stone selective medium to isolate S. aureus. The Kirby-Bauer disk diffusion method was used to determine antibiotic resistance to ampicillin, penicillin, and cefaclor, which represent two distinct classes of antibiotics. The average recovered concentration of respirable heterotrophic organisms found outside each home was 345.38 CFU/m3, with an average of 12.63 CFU/m3 for S. aureus. The average recovered concentration of respirable heterotrophic organisms found inside each home was 460.23 CFU/m3, with an average of 15.39 CFU/m3 for S. aureus. The respirable S. aureus recovered from inside each home had an average resistance of 54.59% to ampicillin and 60.46%. to penicillin. Presence of cefaclor-resistant and of multidrug-resistant S. aureus was the same, averaging 13.20% per house. The respirable S. aureus recovered from outside each home had an average resistance of 34.42% to ampicillin and 41.81% to penicillin. Presence of cefaclor-resistant and of multidrug-resistant S. aureus was the same, averaging 13.96% per house. This study indicates that antibiotic-resistant bioaerosols are commonly found within residential homes. Our results also suggest that resistant strains of airborne culturable S. aureus are present in higher concentrations inside the study homes than outside the homes.
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影响因子：
6.9
作者：
Das, I;Lambert, P;Elliott, T
通讯作者：
Elliott, T
影响因子：
5
作者：
Meklin, T;Reponen, T;Nevalainen, A
通讯作者：
Nevalainen, A
影响因子：
3.6
作者：
Hussain, FM;Boyle-Vavra, S;Daum, RS
通讯作者：
Daum, RS
影响因子：
56.9
作者：
COHEN, ML
通讯作者：
COHEN, ML
影响因子：
6.9
作者：
Cotterill, S;Evans, R;Fraise, AP
通讯作者：
Fraise, AP