A Fork Trap in the Chromosomal Termination Area Is Highly Conserved across All Escherichia coli Phylogenetic Groups.
A Fork Trap in the Chromosomal Termination Area Is Highly Conserved across All Escherichia coli Phylogenetic Groups.
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DOI：
10.3390/ijms22157928
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发表时间：
2021-07-25
影响因子：
5.6
通讯作者：
Rudolph CJ
中科院分区：
文献类型：
--
作者：
Goodall DJ;Jameson KH;Hawkins M;Rudolph CJ
DNA复制的终止，基因组复制的最后阶段，是令人惊讶的复杂性，并且未能使DNA合成达到准确的结论可能会影响基因组稳定性和细胞活力。在大肠杆菌中，终止发生在与起点相对的专门终止区。通过Tus蛋白与基因组ter位点的结合，由单向叉屏障形成“复制叉陷阱”。这种叉状陷阱系统在某些细菌物种中发现，但它似乎不是细菌染色体的一般特征。叉陷阱系统的生化特性已被广泛表征，但对其精确的生理作用知之甚少。在这项研究中，我们比较的位置和分布的ter终止子位点在大肠杆菌。包括志贺氏菌在内的所有系统发生群的大肠杆菌基因组。我们的分析表明所有的ter位点在E.大肠杆菌中，志贺氏菌基因组的变异性略大。我们对Ter位点和Tus蛋白的序列分析表明，叉陷阱可能在所有研究菌株中都是活跃的。此外，我们的分析表明，dif染色体二聚体分辨率网站始终位于最里面的ter网站之间，即使重排改变了最里面的终止区的位置。我们的数据进一步支持复制叉陷阱具有重要的生理作用，提供了进化优势的想法。
Termination of DNA replication, the final stage of genome duplication, is surprisingly complex, and failures to bring DNA synthesis to an accurate conclusion can impact genome stability and cell viability. In Escherichia coli, termination takes place in a specialised termination area opposite the origin. A ‘replication fork trap’ is formed by unidirectional fork barriers via the binding of Tus protein to genomic ter sites. Such a fork trap system is found in some bacterial species, but it appears not to be a general feature of bacterial chromosomes. The biochemical properties of fork trap systems have been extensively characterised, but little is known about their precise physiological roles. In this study, we compare locations and distributions of ter terminator sites in E. coli genomes across all phylogenetic groups, including Shigella. Our analysis shows that all ter sites are highly conserved in E. coli, with slightly more variability in the Shigella genomes. Our sequence analysis of ter sites and Tus proteins shows that the fork trap is likely to be active in all strains investigated. In addition, our analysis shows that the dif chromosome dimer resolution site is consistently located between the innermost ter sites, even if rearrangements have changed the location of the innermost termination area. Our data further support the idea that the replication fork trap has an important physiological role that provides an evolutionary advantage.
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影响因子：
3.5
作者：
Dimude JU;Stein M;Andrzejewska EE;Khalifa MS;Gajdosova A;Retkute R;Skovgaard O;Rudolph CJ
通讯作者：
Rudolph CJ
影响因子：
4.8
作者：
CoskunAri, FF;Hill, TM
通讯作者：
Hill, TM
影响因子：
3.5
作者：
Dimude JU;Midgley-Smith SL;Stein M;Rudolph CJ
通讯作者：
Rudolph CJ
影响因子：
4.6
作者：
Galli, Elisa;Ferat, Jean-Luc;Possoz, Christophe
通讯作者：
Possoz, Christophe
影响因子：
11.4
作者：
Bidnenko, V;Ehrlich, SD;Michel, B
通讯作者：
Michel, B