A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes
复制标题
含有重复序列的新型酵母核孔蛋白 RAT7/NUP159 的条件等位基因导致 mRNA 输出快速停止和核孔复合物可逆聚集
DOI：
--
复制
发表时间：
1995
影响因子：
7.8
通讯作者：
Charles N. Cole
中科院分区：
文献类型：
--
作者：
Lisa C. Gorsch;T. C. Dockendorff;Charles N. Cole
在筛选酿酒酵母核胞质运输信使RNA所需的基因时，我们发现了RAT7基因（核糖核酸运输），该基因编码1460个氨基酸的必需蛋白。Rat7p位于核边缘，呈核孔蛋白的点状分布。此外，Rat7p的中心三分之一包含22个XXFG和3个XFXFG退化重复序列，与迄今为止在大多数酵母和一些哺乳动物核孔蛋白中测序的GLFG和XFXFG重复序列相似。携带温度敏感的rat7-1等位基因的菌株从23℃转移到37℃，导致mRNA输出迅速（在15分钟内）停止，但没有引起携带核定位信号的报告蛋白的细胞质积累。这表明Rat7p可能在核胞质输出RNA中起直接作用。免疫荧光和电镜观察显示，在23℃条件下生长的rat7-1细胞中，大部分核孔复合物（NPCs）聚集在细胞核的一侧。核膜未见超微结构异常。有趣的是，将rat7-1细胞转移到37℃下1小时会导致NPC分散，恢复到接近野生型NPC的分布。温度改变后，免疫荧光不再检测到突变体Rat7p。
In a screen for Saccharomyces cerevisiae genes required for nucleocytoplasmic transport of messenger RNA, we identified the RAT7 gene (ribonucleic acid trafficking), which encodes an essential protein of 1,460 amino acids. Rat7p is located at the nuclear rim in a punctate pattern characteristic of nucleoporins. Furthermore, the central third of Rat7p contains 22 XXFG and three XFXFG degenerate repeats that are similar to signature GLFG and XFXFG repeats present in a majority of yeast and some mammalian nucleoporins sequenced to date. Shift of a strain bearing the temperature-sensitive rat7-1 allele from 23 degrees C to 37 degrees C resulted in rapid (within 15 minutes) cessation of mRNA export, but did not cause concomitant cytoplasmic accumulation of a reporter protein bearing a nuclear localization signal. This suggests that Rat7p may play a direct role in nucleocytoplasmic export of RNA. Immunofluorescence and thin section electron microscopy revealed that in rat7-1 cells grown at 23 degrees C, the majority of nuclear pore complexes (NPCs) were clustered on one side of the nucleus. No ultrastructural abnormalities of the nuclear envelope were seen. Interestingly, shifting rat7-1 cells to 37 degrees C for 1 h caused the NPCs to disperse, restoring near wild-type NPC distribution. After this temperature shift, the mutant Rat7p was no longer detectable by immunofluorescence.
DOI：
10.1016/0889-1605(89)90047-5
发表时间：
1989-08-01
期刊：
JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH
影响因子：
--
作者：
ALLEN, JL;DOUGLAS, MG
通讯作者：
DOUGLAS, MG