Tumor necrosis factor-α induces prostate cancer cell migration in lymphatic metastasis through CCR7 upregulation.
Tumor necrosis factor-α induces prostate cancer cell migration in lymphatic metastasis through CCR7 upregulation.
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DOI：
10.1111/cas.13586
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发表时间：
2018-05
期刊：
影响因子：
5.7
通讯作者：
Mizokami A
中科院分区：
文献类型：
--
作者：
Maolake A;Izumi K;Natsagdorj A;Iwamoto H;Kadomoto S;Makino T;Naito R;Shigehara K;Kadono Y;Hiratsuka K;Wufuer G;Nastiuk KL;Mizokami A
淋巴结转移是前列腺癌预后不良的标志，了解淋巴结转移的机制以及疾病的进一步扩散对于开发新的治疗策略非常重要。最近的研究报道，C-C趋化因子受体7（CCR 7），其配体是CCL 21，在淋巴结转移中大量表达并促进癌症进展。肿瘤坏死因子-α（TNF-α）在肿瘤微环境中以低水平长期产生。本研究的目的是确定TNF-α是否通过激活CCL 21/CCR 7轴促进前列腺癌从转移性淋巴结转移。首先，确定人前列腺癌细胞表达TNF-α和CCR 7。第二，证实低浓度的TNF-α通过ERK的磷酸化诱导前列腺癌细胞中的CCR 7。最后，发现CCL 21通过蛋白激酶p38的磷酸化促进前列腺癌细胞的迁移。我们的研究结果表明，TNF-α导致诱导CCR 7的表达，并且CCL 21/CCR 7轴可能增加前列腺癌细胞在淋巴结转移中的转移潜力。
Understanding the mechanism of lymph node metastasis, a poor prognostic sign for prostate cancer, and the further dissemination of the disease is important to develop novel treatment strategies. Recent studies have reported that C‐C chemokine receptor 7 (CCR7), whose ligand is CCL21, is abundantly expressed in lymph node metastasis and promotes cancer progression. Tumor necrosis factor‐α (TNF‐α) is chronically produced at low levels within the tumor microenvironment. The aim of this study was to determine whether TNF‐α promotes prostate cancer dissemination from metastatic lymph nodes through activation of the CCL21/CCR7 axis. First, human prostate cancer cells were determined to express both TNF‐α and CCR7. Second, low concentrations of TNF‐α were confirmed to induce CCR7 in prostate cancer cells through phosphorylation of ERK. Finally, CCL21 was found to promote the migration of prostate cancer cells through phosphorylation of the protein kinase p38. Our results suggest that TNF‐α leads to the induction of CCR7 expression and that the CCL21/CCR7 axis might increase the metastatic potential of prostate cancer cells in lymph node metastasis.
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DOI：
10.1186/s13046-016-0318-y
发表时间：
2016-03-24
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Hong H;He C;Zhu S;Zhang Y;Wang X;She F;Chen Y
通讯作者：
Chen Y
影响因子：
9
作者：
通讯作者：
--
影响因子：
254.7
作者：
Siegel, Rebecca;Ma, Jiemin;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin
影响因子：
3.4
作者：
Kadono, Yoshifumi;Nohara, Takahiro;Namiki, Mikio
通讯作者：
Namiki, Mikio
影响因子：
--
作者：
Izumi K;Mizokami A;Lin HP;Ho HM;Iwamoto H;Maolake A;Natsagdorj A;Kitagawa Y;Kadono Y;Miyamoto H;Huang CK;Namiki M;Lin WJ
通讯作者：
Lin WJ