Techniques used to study the DNA polymerase reaction pathway.

Techniques used to study the DNA polymerase reaction pathway.
复制标题

DOI:
10.1016/j.bbapap.2009.07.021
复制
发表时间:
2010-05
期刊:
Biochimica et biophysica acta
影响因子:
--
通讯作者:
Joyce CM
Joyce CM
中科院分区:
其他
文献类型:
--
作者:
Joyce CM

文献摘要

参考文献

被引文献

相似文献

DNA聚合酶的最小反应途径是在20多年前使用化学淬灭方法建立的。从那时起,人们对反应途径中的非共价步骤产生了相当大的兴趣,涉及聚合酶或其DNA底物的构象变化可能在底物特异性中发挥作用。为了研究这些构象转变,已经设计了基于双折射的测定,并且所获得的结果为反应途径增加了新的细节。
A minimal reaction pathway for DNA polymerases was established over 20 years ago using chemical quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.
DOI: 10.1038/nsmb.1540
发表时间: 2009-02
影响因子: 16.8
作者:
通讯作者: --
DOI: 10.1021/bi7021848
发表时间: 2008-06-10
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
Joyce, Catherine M.;Potapova, Olga;Grindley, Nigel D. F.
通讯作者: Grindley, Nigel D. F.
DOI: 10.1073/pnas.0608952104
发表时间: 2007-03-13
影响因子: 11.1
作者:
Castro, Christian;Smidansky, Eric;Cameron, Craig E.
通讯作者: Cameron, Craig E.
DOI: 10.1021/bi00399a057
发表时间: 1987-12-15
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
KUCHTA, RD;MIZRAHI, V;BENKOVIC, SJ
通讯作者: BENKOVIC, SJ
DOI: 10.1021/bi002176j
发表时间: 2001-05-08
期刊: BIOCHEMISTRY
影响因子: 2.9
作者:
Arndt, JW;Gong, WM;Chan, MK
通讯作者: Chan, MK