Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans.
Single-cell genomics of multiple uncultured stramenopiles reveals underestimated functional diversity across oceans.
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多种未经培养的杂质的单细胞基因组学揭示了整个海洋中低估的功能多样性。
DOI：
10.1038/s41467-017-02235-3
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发表时间：
2018-01-22
影响因子：
16.6
通讯作者：
Wincker P
中科院分区：
文献类型：
--
作者：
Seeleuthner Y;Mondy S;Lombard V;Carradec Q;Pelletier E;Wessner M;Leconte J;Mangot JF;Poulain J;Labadie K;Logares R;Sunagawa S;de Berardinis V;Salanoubat M;Dimier C;Kandels-Lewis S;Picheral M;Searson S;Tara Oceans Coordinators;Pesant S;Poulton N;Stepanauskas R;Bork P;Bowler C;Hingamp P;Sullivan MB;Iudicone D;Massana R;Aury JM;Henrissat B;Karsenti E;Jaillon O;Sieracki M;de Vargas C;Wincker P
单细胞真核生物（原生生物）是全球海洋营养物质和能量生物地球化学循环的关键参与者。虽然它们作为初级生产者和食草动物的作用得到了很好的理解，但由于在培养和测序其自然多样性方面的挑战，它们生活史的其他方面仍然不清楚。在这里，我们利用单细胞基因组学和宏基因组学数据从环球塔拉海洋考察分析基因组内容和明显的海洋分布的七个流行的血统未培养的异养层棘。根据现有数据，每一个测序的基因组或基因型似乎都有一个特定的海洋分布，主要与水温和深度有关。基因组内容提供了细胞运动，食物谱和营养阶段，包括从原核生物水平基因转移对他们的生活方式的潜在影响方面的专业化的假设。我们的研究结果支持的想法，突出的异养海洋原生生物在海洋生态中执行不同的功能。许多海洋原生生物的生物学，如层虫，仍然不清楚。在这里，作者利用单细胞基因组学和宏基因组学分析基因组内容和明显的海洋分布的七个流行的血统的未培养的异养stratifiles。
Single-celled eukaryotes (protists) are critical players in global biogeochemical cycling of nutrients and energy in the oceans. While their roles as primary producers and grazers are well appreciated, other aspects of their life histories remain obscure due to challenges in culturing and sequencing their natural diversity. Here, we exploit single-cell genomics and metagenomics data from the circumglobal Tara Oceans expedition to analyze the genome content and apparent oceanic distribution of seven prevalent lineages of uncultured heterotrophic stramenopiles. Based on the available data, each sequenced genome or genotype appears to have a specific oceanic distribution, principally correlated with water temperature and depth. The genome content provides hypotheses for specialization in terms of cell motility, food spectra, and trophic stages, including the potential impact on their lifestyles of horizontal gene transfer from prokaryotes. Our results support the idea that prominent heterotrophic marine protists perform diverse functions in ocean ecology. The biology of many marine protists, such as stramenopiles, remains obscure. Here, the authors exploit single-cell genomics and metagenomics to analyze the genome content and apparent oceanic distribution of seven prevalent lineages of uncultured heterotrophic stramenopiles.
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影响因子：
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作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
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Lima-Mendez, Gipsi;Faust, Karoline;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
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通讯作者：
Gorsky G