Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars.
Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars.
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DOI：
10.1016/j.bone.2010.02.019
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发表时间：
2010-06
期刊：
影响因子：
4.1
通讯作者：
Mina, Mina
中科院分区：
文献类型：
--
作者：
Balic, Anamaria;Aguila, H. Leonardo;Caimano, Melissa J.;Francone, Victor P.;Mina, Mina
在过去的几年里，在包括牙髓在内的牙科组织中鉴定可能的干细胞也被称为“间充质干细胞”(MSC)方面取得了重大进展。目前认为牙髓中的MSC与从其他组织中分离的MSC有一定的相似性。然而，牙髓中的细胞特征仍然很差。本研究重点研究萌出和未萌出小鼠磨牙牙髓中祖细胞和干细胞的特征。我们的研究表明，未萌出磨牙的牙髓中含有大量表达CD90+/CD45-、CD117+/CD45-、SCA-1+/CD45-的细胞，CD45+细胞很少。我们的体外功能研究表明，未萌出磨牙的牙髓细胞具有广泛的成骨潜能，但不能分化为软骨细胞和脂肪细胞。与未萌出磨牙相比，萌出磨牙牙髓细胞数量减少，CD45+细胞比例增加，CD90+/CD45-、CD117+/CD45-细胞比例降低。体外功能分析表明，一小部分细胞具有分化为成牙本质细胞、成骨细胞、脂肪细胞和软骨细胞的能力。与未萌出的磨牙相比，萌出磨牙的牙髓细胞的成骨能力显著降低。此外，萌出磨牙牙髓细胞的成脂和成软骨分化依赖于较长的诱导期和罕见的诱导期。根据这些发现，我们认为萌出磨牙的牙髓含有少量的多能细胞，而未萌出的磨牙的牙髓不包含多能细胞，但富含参与形成冠状和根状成牙本质细胞的骨牙本质祖细胞。
In the past few years there have been significant advances in the identification of putative stem cells also referred to as “mesenchymal stem cells” (MSC) in dental tissues including the dental pulp. It is thought that MSC in dental pulp share certain similarities with MSC isolated from other tissues. However, cells in dental pulp are still poorly characterized. This study focused on the characterization of progenitor and stem cells in dental pulps of erupted and unerupted mice molars. Our study showed that dental pulps from unerupted molars contain a significant number of cells expressing CD90+/CD45-, CD117+/CD45-, Sca-1+/CD45- and little if any CD45+ cells. Our in vitro functional studies showed that dental pulp cells from unerupted molars displayed extensive osteo-dentinogenic potential but were unable to differentiate into chondrocytes and adipocytes. Dental pulp from erupted molars displayed a reduced number of cells, contained higher percentage of CD45+ and lower percentage of cells expressing CD90+/CD45-, CD117+/CD45- as compared to unerupted molars. In vitro functional assays demonstrated the ability of a small fraction of cells to differentiate into odontoblasts, osteoblasts, adipocytes and chondrocytes. There was a significant reduction in the osteo-dentinogenic potential of the pulp cells derived from erupted molars compared to unerupted molars. Furthermore, the adipogenic and chondrogenic differentiation of pulp cells from erupted molars was dependent on a long induction period and infrequent. Based on these findings we propose that the dental pulp of the erupted molars contain a small population of multipotent cells, whereas the dental pulp of the unerupted molars does not contain multipotent cells but is enriched in osteo-dentinogenic progenitors engaged in the formation of coronal and radicular odontoblasts.
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影响因子：
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通讯作者：
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影响因子：
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