Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.
Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.
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DOI：
10.1083/jcb.200908150
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发表时间：
2010-01-11
期刊：
影响因子：
--
通讯作者：
Scholey JM
中科院分区：
文献类型：
--
作者：
Civelekoglu-Scholey G;Tao L;Brust-Mascher I;Wollman R;Scholey JM
核被膜层蛋白B通过抑制反作用力驱动蛋白的竞争运动来稳定纺锤体微管。我们测试了经典的假设，即星形，前中期双极有丝分裂纺锤体保持平衡的外向和内向的力量施加在纺锤体极驱动蛋白-5和-14在体外和体内的数据从果蝇胚胎建模。在整个前中期，斑点的两个电机对齐极间微管（MTs [ipMTs]），和电机扰动改变纺锤体长度，如预测。纯化的驱动蛋白-5和-14的竞争运动性很好地描述了一个随机的，相反的动力冲程模型，结合电机动力学和负载依赖性脱离。从这个模型的电机参数被施加到一个新的随机力平衡模型的前中期纺锤体，提供了一个很好的适合胚胎的数据。虚拟纺锤体的维持需要动态ipMT和驱动蛋白-5到驱动蛋白-14的比率与胚胎中发现的比率相匹配的窄范围。功能扰动和建模表明，这一范围可以显着扩展的拆卸层蛋白-B信封，周围的前中期纺锤体和增强微调，拮抗驱动蛋白力的平衡，以保持强大的前中期纺锤体作为MT组装和染色体被推到赤道。
The lamin-B nuclear envelope stabilizes spindle microtubules by keeping the competitive motility of opposing-force kinesins in check. We tested the classical hypothesis that astral, prometaphase bipolar mitotic spindles are maintained by balanced outward and inward forces exerted on spindle poles by kinesin-5 and -14 using modeling of in vitro and in vivo data from Drosophila melanogaster embryos. Throughout prometaphase, puncta of both motors aligned on interpolar microtubules (MTs [ipMTs]), and motor perturbation changed spindle length, as predicted. Competitive motility of purified kinesin-5 and -14 was well described by a stochastic, opposing power stroke model incorporating motor kinetics and load-dependent detachment. Motor parameters from this model were applied to a new stochastic force-balance model for prometaphase spindles, providing a good fit to data from embryos. Maintenance of virtual spindles required dynamic ipMTs and a narrow range of kinesin-5 to kinesin-14 ratios matching that found in embryos. Functional perturbation and modeling suggest that this range can be extended significantly by a disassembling lamin-B envelope that surrounds the prometaphase spindle and augments the finely tuned, antagonistic kinesin force balance to maintain robust prometaphase spindles as MTs assemble and chromosomes are pushed to the equator.
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影响因子：
9.2
作者：
Goshima, G;Wollman, R;Vale, RD
通讯作者：
Vale, RD
影响因子：
3.7
作者：
Cahu, Julie;Olichon, Aurelien;Hentrich, Christian;Schek, Henry;Drinjakovic, Jovana;Zhang, Cunjie;Doherty-Kirby, Amanda;Lajoie, Gilles;Surrey, Thomas
通讯作者：
Surrey, Thomas
影响因子：
7.8
作者：
Cullen, CF;Deák, P;Ohkura, H
通讯作者：
Ohkura, H
DOI：
10.1083/jcb.135.2.399
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gaglio T;Saredi A;Bingham JB;Hasbani MJ;Gill SR;Schroer TA;Compton DA
通讯作者：
Compton DA
影响因子：
7.8
作者：
Cottingham, FR;Gheber, L;Hoyt, MA
通讯作者：
Hoyt, MA