Activity-dependent dendritic spine shrinkage and growth involve downregulation of cofilin via distinct mechanisms.
Activity-dependent dendritic spine shrinkage and growth involve downregulation of cofilin via distinct mechanisms.
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DOI：
10.1371/journal.pone.0094787
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Halpain S
中科院分区：
文献类型：
--
作者：
Calabrese B;Saffin JM;Halpain S
目前的模型认为，依赖于cofilin的肌动蛋白切断对树突脊柱体积产生负面影响。研究表明，增加的cofilin活性是活动依赖性脊柱萎缩的基础，而减少的cofilin活性诱导活动依赖性脊柱生长。相反，我们认为这两种类型的结构可塑性都与cofilin活性的降低有关。然而，抑制的机制决定了脊柱形态学的结果。大鼠海马培养的RNAi表明，cofilin对正常脊柱维持至关重要。在活动依赖性脊柱萎缩的早期阶段，Cofilin-F-actin结合和丝刺端产生减少；Cofilin浓度也降低。组织蛋白酶B/L家族的抑制可防止cofilin丢失和脊柱收缩。相反，在活动依赖性脊柱生长过程中，LIM激酶刺激cofilin磷酸化，从而激活磷脂酶D-1，促进肌动蛋白聚合。这些结果暗示了新的分子机制，并提示对当前的cofilin如何在活性依赖的结构可塑性中起作用的模型进行修订。
A current model posits that cofilin-dependent actin severing negatively impacts dendritic spine volume. Studies suggested that increased cofilin activity underlies activity-dependent spine shrinkage, and that reduced cofilin activity induces activity-dependent spine growth. We suggest instead that both types of structural plasticity correlate with decreased cofilin activity. However, the mechanism of inhibition determines the outcome for spine morphology. RNAi in rat hippocampal cultures demonstrates that cofilin is essential for normal spine maintenance. Cofilin-F-actin binding and filament barbed-end production decrease during the early phase of activity-dependent spine shrinkage; cofilin concentration also decreases. Inhibition of the cathepsin B/L family of proteases prevents both cofilin loss and spine shrinkage. Conversely, during activity-dependent spine growth, LIM kinase stimulates cofilin phosphorylation, which activates phospholipase D-1 to promote actin polymerization. These results implicate novel molecular mechanisms and prompt a revision of the current model for how cofilin functions in activity-dependent structural plasticity.
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影响因子：
21.3
作者：
Gohla, A;Birkenfeld, J;Bokoch, GM
通讯作者：
Bokoch, GM
DOI：
10.1523/jneurosci.1746-10.2010
发表时间：
2010-09-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Fortin DA;Davare MA;Srivastava T;Brady JD;Nygaard S;Derkach VA;Soderling TR
通讯作者：
Soderling TR
影响因子：
16.2
作者：
Frost, Nicholas A.;Shroff, Hari;Kong, Huihui;Betzig, Eric;Blanpied, Thomas A.
通讯作者：
Blanpied, Thomas A.
影响因子：
25
作者：
Gu, Jiaping;Lee, Chi Wai;Fan, Yanjie;Komlos, Daniel;Tang, Xin;Sun, Chicheng;Yu, Kuai;Hartzell, H. Criss;Chen, Gong;Bamburg, James R.;Zheng, James Q.
通讯作者：
Zheng, James Q.
影响因子：
16
作者：
Andrianantoandro, Ernesto;Pollard, Thomas D.
通讯作者：
Pollard, Thomas D.