Genomic study of RNA polymerase II and III SNAPc-bound promoters reveals a gene transcribed by both enzymes and a broad use of common activators.
Genomic study of RNA polymerase II and III SNAPc-bound promoters reveals a gene transcribed by both enzymes and a broad use of common activators.
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RNA聚合酶II和III SNAPC结合启动子的基因组研究揭示了酶转录的基因和广泛使用共同活化剂。
DOI：
10.1371/journal.pgen.1003028
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Hernandez N
中科院分区：
文献类型：
--
作者：
James Faresse N;Canella D;Praz V;Michaud J;Romascano D;Hernandez N
SNAPc是RNA聚合酶（pol） II和pol III使用的少数基础转录因子之一。为了确定人类细胞中活性SNAPc依赖启动子的集合，我们在全基因组定位了四个SNAPc亚基，GTF2B (TFIIB), BRF2， pol II和pol III。在SNAPc和其他因子占据的约70个位点中，包括pol II snRNA基因、带3型启动子的pol III基因和少数未注释的位点，大多数主要由pol II和GTF2B或pol III和BRF2占据。一个值得注意的例外是RPPH1基因，它被大量的两种聚合酶占据。我们发现绝大多数依赖snapc的启动子在其增强子区域招募POU2F1和/或ZNF143，并且一个子集还招募GABP，这是一个与snapc依赖的转录有关的新因子。这些激活因子与G1中的pol II和III启动子结合的时间稍早于聚合酶，而ZNF143是高效转录起始复合物组装所必需的。结果表征了一组具有独特性质的基因，并确定聚合酶的特异性在体内不是绝对的。依赖snapc的启动子在细胞启动子中是独一无二的，它们彼此非常相似，尽管其中一些启动子募集RNA聚合酶II和其他RNA聚合酶III。我们检查了人类基因组中存在的所有与snapc结合的启动子。我们发现它们的数量少得惊人，只有大约70个启动子。其中，绝大多数与预期的RNA聚合酶II或RNA聚合酶III结合，但迄今为止被认为是RNA聚合酶III基因的一个基因也被显著水平的RNA聚合酶II所占据。RNA聚合酶II和RNA聚合酶III依赖snapc的启动子都使用大量重叠的几种转录激活因子，包括GABP，这是一种涉及snRNA基因转录的新因子。
SNAPc is one of a few basal transcription factors used by both RNA polymerase (pol) II and pol III. To define the set of active SNAPc-dependent promoters in human cells, we have localized genome-wide four SNAPc subunits, GTF2B (TFIIB), BRF2, pol II, and pol III. Among some seventy loci occupied by SNAPc and other factors, including pol II snRNA genes, pol III genes with type 3 promoters, and a few un-annotated loci, most are primarily occupied by either pol II and GTF2B, or pol III and BRF2. A notable exception is the RPPH1 gene, which is occupied by significant amounts of both polymerases. We show that the large majority of SNAPc-dependent promoters recruit POU2F1 and/or ZNF143 on their enhancer region, and a subset also recruits GABP, a factor newly implicated in SNAPc-dependent transcription. These activators associate with pol II and III promoters in G1 slightly before the polymerase, and ZNF143 is required for efficient transcription initiation complex assembly. The results characterize a set of genes with unique properties and establish that polymerase specificity is not absolute in vivo. SNAPc-dependent promoters are unique among cellular promoters in being very similar to each other, even though some of them recruit RNA polymerase II and others RNA polymerase III. We have examined all SNAPc-bound promoters present in the human genome. We find a surprisingly small number of them, some 70 promoters. Among these, the large majority is bound by either RNA polymerase II or RNA polymerase III, as expected, but one gene hitherto considered an RNA polymerase III gene is also occupied by significant levels of RNA polymerase II. Both RNA polymerase II and RNA polymerase III SNAPc-dependent promoters use a largely overlapping set of a few transcription activators, including GABP, a novel factor implicated in snRNA gene transcription.
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模因套件：用于发现和搜索的工具。
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