Cell Anchorage Permits Efficient Signal Transduction Between Ras and Its Downstream Kinases*
Cell Anchorage Permits Efficient Signal Transduction Between Ras and Its Downstream Kinases*
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细胞锚定允许 Ras 及其下游激酶之间有效的信号转导*
DOI：
10.1074/jbc.272.14.8849
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Rudy L. Juliano
中科院分区：
文献类型：
--
作者：
T. Lin;Qiming Chen;A. Howe;Rudy L. Juliano
细胞锚定强烈影响由肽有丝分裂原启动的信号转导级联。对于表皮生长因子和血小板衍生生长因子，当细胞保持悬浮时，与锚定到纤连蛋白基质的细胞相比，共有丝裂原活化蛋白激酶级联的活化受损。信号级联中的上游事件，包括有丝分裂原受体的酪氨酸磷酸化和Ras的GTP负载，在锚定和悬浮细胞中是相似的。然而，在悬浮细胞中，信号向Raf和随后向下游激酶MEK和促分裂原活化蛋白激酶的传播显著减弱。因此，在由肽有丝分裂原启动的信号级联中，Ras和Raf之间似乎存在明显的锚定依赖性步骤。这些观察可能有重要的意义，了解细胞生长的锚定依赖性。
Cell anchorage strongly affects the signal transduction cascade initiated by peptide mitogens. For both epidermal growth factor and platelet-derived growth factor, activation of the consensus mitogen-activated protein kinase cascade is impaired when cells are held in suspension as compared with cells anchored to a fibronectin substratum. Upstream events in the signaling cascade, including tyrosine phosphorylation of the mitogen receptor and GTP loading of Ras, are similar in anchored and suspended cells. However, propagation of the signal to Raf and subsequently to the downstream kinases MEK and mitogen-activated protein kinase is markedly attenuated in suspended cells. Thus, there seems to be a distinct anchorage-dependent step between Ras and Raf in the signaling cascade initiated by peptide mitogens. These observations may have important implications for understanding the anchorage dependence of cell growth.
影响因子：
56.9
作者：
VUORI, K;RUOSLAHTI, E
通讯作者：
RUOSLAHTI, E