Expression and ultrastructural localization of Mint2 in the spinal cord of rats
Expression and ultrastructural localization of Mint2 in the spinal cord of rats
复制标题
Mint2在大鼠脊髓中的表达及超微结构定位
DOI：
10.1007/s11033-010-0153-8
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发表时间：
2010
影响因子：
2.8
通讯作者：
Huang, Jing
中科院分区：
文献类型：
--
作者：
Yao, Ling-Ling;Wang, Yong-Gang;Liu, Xue-Yuan;Jin, Ji-Yang;Tao, Bei-Bei;Chen, Yu-Juan;Yu, Yong-Chun;Bian, Wei-Hong;Yu, Jing;Huang, Jing
作为接头分子的薄荷蛋白家族包含3个成员：Mint1、Mint2和Mint3。尽管Mint3广泛表达，但Mint1和Mint2仅在神经元中特异表达。在这里，我们展示了Mint1和Mint2在大鼠脊髓中的表达模式。免疫印迹结果显示，Mint2在大鼠脊髓中的蛋白水平高于Mint1。为了解Mint2在大鼠脊髓中的分布，采用原位杂交技术，发现Mint2在大鼠脊髓颈、胸、腰段广泛分布，在运动神经元中检测到高强度信号。荧光免疫组织化学进一步证实，Mint2也存在于灰质特别是运动神经元中，其中Mint2主要位于核周，而Mint1相对较低。电镜观察发现，Mint2主要定位于腰段运动神经元核周的小泡内，同时也定位于髓鞘轴突和突触前终末。这些数据表明，Mint2可能比其他两个家族成员在脊髓中发挥更重要的作用。
Mint protein family, as adaptor molecules, contains three members, Mint1, Mint2 and Mint3. Although Mint3 is ubiquitously expressed, Mint1 and Mint2 have been reported to express specifically in neuron. Here we demonstrated Mint1 and Mint2 expression pattern in rat spinal cord. The protein level of Mint2 was found to be higher than that of Mint1 in rat spinal by western blot. In an attempt to know Mint2 distribution in the spinal cord of rat, in situ hybridization was carried out, Mint2 mRNA was showed to be ubiquitously distributed in cervical, thoracic and lumbar sections of rat spinal cord, and high intensive signal was detected in motor neurons. These were further confirmed by fluorescent immunohistochemistry, Mint2 was also found to exist throughout gray matter especially motor neurons where Mint2 was mainly located in perikaryon, however, Mint1 was showed to be relatively lower. By electron microscope, Mint2 was found to be mainly located in vesicles in perikaryon in motor neuron of lumbar section, and at the same time Mint2 was located in axons in myelin and presynaptic terminals. These data suggest that Mint2 may play more important role in spinal cord than the other two family members.
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影响因子：
3.4
作者：
M. Okamoto;T. Matsuyama;M. Sugita
通讯作者：
M. Okamoto;T. Matsuyama;M. Sugita
影响因子：
5.3
作者：
J. Borg;J. Ooi;E. Levy;B. Margolis
通讯作者：
J. Borg;J. Ooi;E. Levy;B. Margolis
日本壁虎脑蛋白44样(Brp44l)基因的分子特征及其断尾后脊髓中的表达变化
影响因子：
2.8
作者：
Jiang, Maorong;Gu, Xiaosong;Liu, Yan
通讯作者：
Liu, Yan
DOI：
10.1073/pnas.252774899
发表时间：
2003-02-04
影响因子：
11.1
作者：
Ho, A;Morishita, W;Südhof, TC
通讯作者：
Südhof, TC
影响因子：
4.8
作者：
Okamoto, M;Sudhof, TC
通讯作者：
Sudhof, TC