Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis.
Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis.
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DOI：
10.4142/jvs.2018.19.1.79
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发表时间：
2018-01-31
影响因子：
1.8
通讯作者：
Nagahata H
中科院分区：
文献类型：
--
作者：
Gondaira S;Higuchi H;Iwano H;Nishi K;Nebu T;Nakajima K;Nagahata H
支原体属是传染性细菌，支原体乳腺炎是奶牛场的严重生产问题。牛乳腺上皮细胞（bMEC）在消除病原体中具有重要作用，但牛支原体对bMEC的影响尚未完全描述。目的：阐明小鼠抗乳腺内支原体感染的免疫应答。牛，我们进行了微阵列分析，以检查和分析用M刺激后bMEC的mRNA表达。牛我们还比较了M.牛、金黄色葡萄球菌和大肠杆菌对bMEC中免疫相关mRNA表达的影响。转录组分析表明，mRNA编码赖氨酸特异性脱甲基酶4D的水平显着降低，表明免疫反应受到组蛋白脱甲基酶活性降低的抑制。在热灭活M刺激的bMEC中，白细胞介素（IL）-1β、IL-6、肿瘤坏死因子α、toll样受体（TLR）2和TLR 4 mRNA表达水平显著升高。bovis的表达水平，但低于热灭活S.金黄色葡萄球菌E.杆菌我们的结果表明，M. bovis对bMEC mRNA表达的影响弱于E. coli或S.金黄色。此外，活M。bovis可诱导bMEC中免疫应答的抑制。
Mycoplasma spp. are contagious bacteria, and mycoplasmal mastitis is a serious productivity problem on dairy farms. Bovine mammary epithelial cells (bMECs) have an important role in the elimination of pathogens, but the effect of Mycoplasma bovis on bMECs has not been fully described. To elucidate the immune response against intramammary infection by M. bovis, we undertook microarray analysis to examine and profile mRNA expression in bMECs after stimulation with M. bovis. We also compared the effects of M. bovis, Staphylococcus aureus, and Escherichia coli on immune-related mRNA expression in bMECs. Transcriptome analysis indicated a significant decrease in the level of mRNA-encoding lysine-specific demethylase 4D, suggesting that the immune response is suppressed by a decrease in histone demethylase activity. Interleukin (IL)-1β, IL-6, tumor necrosis factor alpha, toll-like receptor (TLR) 2, and TLR4 mRNA expression levels were significantly increased in bMECs stimulated with heat-killed M. bovis, but the expression levels were lower than those following stimulation by heat-killed S. aureus or E. coli. Our results suggest that M. bovis weakly affects mRNA expression in bMECs compared to the effects of E. coli or S. aureus. Moreover, live M. bovis may induce suppression of the immune response in bMECs.
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影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
Rainard P
DOI：
10.1186/1297-9686-43-24
发表时间：
2011-06-24
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Brand B;Hartmann A;Repsilber D;Griesbeck-Zilch B;Wellnitz O;Kühn C;Ponsuksili S;Meyer HH;Schwerin M
通讯作者：
Schwerin M
影响因子：
4.4
作者：
Shio，Marina T.;Hassan，Ghada S.;Mourad，Walid
通讯作者：
Mourad，Walid
影响因子：
3.5
作者：
Griesbeck-Zilch, B.;Meyer, H. H. D.;Wellnitz, O.
通讯作者：
Wellnitz, O.
影响因子：
3.1
作者：
Guenther, Juliane;Esch, Kathrin;Seyfert, Hans-Martin
通讯作者：
Seyfert, Hans-Martin