Pimpinellin Inhibits Collagen-induced Platelet Aggregation and Activation Through Inhibiting Granule Secretion and PI3K/Akt Pathway.
Pimpinellin Inhibits Collagen-induced Platelet Aggregation and Activation Through Inhibiting Granule Secretion and PI3K/Akt Pathway.
复制标题
Pimpinellin 通过抑制颗粒分泌和 PI3K/Akt 途径来抑制胶原蛋白诱导的血小板聚集和激活
DOI：
10.3389/fphar.2021.706363
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Qin Z
中科院分区：
文献类型：
--
作者：
Liu G;Yuan Z;Tian X;Xiong X;Guo F;Lin Z;Qin Z
茴芹素是从飞龙掌血的根中提取的香豆素类化合物。其对血小板功能的影响尚未研究。这项研究发现，茴芹素预处理有效地抑制胶原诱导的血小板聚集，但不改变ADP和凝血酶诱导的聚集。经茴芹素预处理的血小板α颗粒（CD 62）水平降低，致密颗粒（ATP释放）分泌减少。匹匹那林处理的血小板也表现出降低的凝块反应和TxB 2的产生。茴芹素预处理抑制人血小板在纤维蛋白原涂覆表面上的粘附和铺展。对施用茴芹素（40 mg/kg）的小鼠的尾部出血时间的分析显示，茴芹素没有显著改变尾部出血时间、血细胞数量以及APTT和PT水平。茴芹素抑制胶原诱导的小鼠血小板体外聚集。免疫印迹结果显示，茴芹素抑制胶原诱导的血小板PI 3 K-Akt-Gsk 3 β磷酸化和PKC/MAPK。
Pimpinellin is a coumarin-like compound extracted from the root of Toddalia asiatica. Its effects on platelet function has not been investigated. This study found that pimpinellin pretreatment effectively inhibited collagen-induced platelet aggregation, but did not alter ADP- and thrombin-induced aggregation. Platelets pretreated with pimpinellin showed reduced α granule (CD62) level and secretion of dense granule (ATP release). Pimpinellin-treated platelets also exhibited decreased clot reaction and TxB2 production. Pimpinellin pretreatment suppressed adhesion and spreading of human platelets on the fibrinogen coated surface. Analysis of tail bleeding time of mice administered with pimpinellin (40 mg/kg) revealed that pimpinellin did not change tail bleeding time significantly, number of blood cells, and APTT and PT levels. Pimpinellin inhibited collagen-induced ex vivo aggregation of mice platelets. Immunoblotting results showed that pimpinellin suppressed collagen-induced phosphorylation of PI3K-Akt-Gsk3β and PKC/MAPK in platelets.
登录
查看更多内容
影响因子：
6.1
作者：
Hanai, Yoshiteru;Adachi, Seiji;Tokuda, Haruhiko
通讯作者：
Tokuda, Haruhiko
DOI：
10.1155/2013/861786
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Fuentes E;Palomo I
通讯作者：
Palomo I
影响因子：
7.4
作者：
Blair P;Flaumenhaft R
通讯作者：
Flaumenhaft R
影响因子：
3.8
作者：
Ginsberg MH
通讯作者：
Ginsberg MH
DOI：
10.1007/s12265-013-9529-1
发表时间：
2014-02-01
影响因子：
3.4
作者：
Fontana, P.;Zufferey, A.;Reny, J. -L.
通讯作者：
Reny, J. -L.