Ultracytochemical study of medullary bone calcification in estrogen injected male Japanese quail
Ultracytochemical study of medullary bone calcification in estrogen injected male Japanese quail
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注射雌激素雄性日本鹌鹑髓质骨钙化的超细胞化学研究
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
A. Hirata
中科院分区：
文献类型：
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作者：
Toshio Yamamoto;Hiroaki Nakamura;T. Tsuji;A. Hirata
通过使用雌激素诱导的雄性日本鹌鹑的髓质骨，进行精细结构和细胞化学研究，以阐明髓质骨钙化的模式，特别是与硫酸化糖胺聚糖的关系。在雌激素处理后4天和7天收集胫骨。髓骨向内向骨髓腔发育，皮质骨附近和小梁较深部分开始钙化，在小梁的延伸尖端和表面区域伴有宽的类骨质。通过高二胺铁 (HID) 检测到的硫酸化糖胺聚糖以类似于小梁钙化基质的模式分布在基质中。皮质骨经 HID 染色呈阴性。在未脱钙的标本中，在钙化区域可见钙化结节。在脱钙样本的基质中还观察到由细丝状材料、边缘致密层和中央核心组成的球状结构。钙化结节和球状结构均表现出相同的分布模式，即它们分散在表面区域并在基质的深层区域聚结。 HID-硫代碳酰肼-银蛋白 (HID-TCH-SP) 染色显示球状结构完全呈阳性，表明硫酸化糖胺聚糖的定位。这些结果强烈表明髓质骨钙化是通过钙化结节的聚结而进展的，并且硫酸化糖胺聚糖在球状钙化的调节中发挥着重要作用。 Anat Rec 264:25–31, 2001。© 2001 Wiley-Liss, Inc.
Fine structural and cytochemical studies were performed to clarify the pattern of medullary bone calcification, specifically in relation to sulfated glycosaminoglycans, by using estrogen‐induced medullary bone of male Japanese quails. Tibiae were collected at 4 and 7 days after estrogen treatment. Medullary bone had developed inward toward the marrow cavity, and calcification had begun near the cortical bone and deeper parts of the trabeculae, accompanied by wide osteoid at extending tips and surface areas of the trabeculae. Sulfated glycosaminoglycans, detected by high iron diamine (HID), were distributed in the matrix in a pattern similar to that of calcified matrix of the trabeculae. Cortical bone was negatively stained by HID. In undecalcified specimens, calcified nodules were seen in areas undergoing calcification. Globular structures composed of fine filamentous materials, a marginal dense layer, and central core, were also observed in the matrix of decalcified specimens. Both the calcified nodules and globular structures showed the same distribution pattern, i.e., they were dispersed at surface areas and coalesced in the deeper areas of the matrix. The globular structures were exclusively positive for HID–thiocarbohydrazide–silver protein (HID‐TCH‐SP) stain, indicating the localization of sulfated glycosaminoglycans. These results strongly suggest that medullary bone calcification progresses by the coalescence of calcified nodules and that sulfated glycosaminoglycans play an important role for the regulation of globular calcification. Anat Rec 264:25–31, 2001. © 2001 Wiley‐Liss, Inc.
日本鹌鹑髓质骨相关蛋白多糖的体外合成。
影响因子：
3.9
作者：
Hunter,SJ;Schraer,H
通讯作者：
Schraer,H