Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds
Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds
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DAM簇的热响应遗传和表观遗传调控控制桃花蕾的休眠和冷需
DOI：
10.1038/s41438-020-0336-y
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发表时间：
2020-08
影响因子：
8.7
通讯作者：
Liu Zongrang
中科院分区：
文献类型：
--
作者：
Zhu Hong;Chen Pao-Yang;Zhong Silin;Dardick Chris;Callahan Ann;An Yong-Qiang;van Knocker Steve;Yang Yingzhen;Zhong Gan-Yuan;Abbott Albert;Liu Zongrang
桃休眠相关的MADS-box（DAM）基因簇是桃休眠发生和结束、低温反应和花芽发育的关键调控中心。然而，不同的温度制度如何与六个相连的DAM基因相互作用和调节仍然不清楚。在这里，我们表明，低温下调DAM 1和DAM 3 -6在休眠的花芽具有不同的模式，并确定DAM 4作为最丰富的表达。我们揭示了多个表观遗传事件，三甲基组蛋白H3赖氨酸27（H3 K27 me 3）诱导的寒冷特异性在DAM 1和DAM 5，21-nt的sRNA在DAM 3和诱导的ncRNA在DAM 4。这种诱导与其同源DAM的下调呈负相关。我们还发现，6个DAM是高甲基化的，与24-nt sRNA的产生有关。因此，不同表观遗传元件的冷冻响应动态及其相互作用可能定义了每个DAM的不同表达丰度和下调模式。我们进一步表明，五种DAM的表达在冷却后的温暖温度下保持稳定不变或持续下调，这种调节状态与sRNA表达，H3 K27 me 3和CHH甲基化的强烈增加相关，这在DAM 4中特别明显。这种抑制性表观遗传标记的强烈增加可能不可逆地加强DAM的冷冻抑制，以确保花发育编程不受任何残留的DAM抑制。总之，我们揭示了桃DAM簇的遗传和表观遗传调控的新信息，这将是基础性的意义，了解潜在的调控机制的需冷量和休眠解除，并在实际应用中提高开花时间和萌芽的可塑性，以适应不断变化的气候。
The Dormancy-associated MADS-box (DAM) gene cluster in peach serves as a key regulatory hub on which the seasonal temperatures act and orchestrate dormancy onset and exit, chilling response and floral bud developmental pace. Yet, how different temperature regimes interact with and regulate the six linked DAM genes remains unclear. Here, we demonstrate that chilling downregulates DAM1 and DAM3–6 in dormant floral buds with distinct patterns and identify DAM4 as the most abundantly expressed one. We reveal multiple epigenetic events, with tri-methyl histone H3 lysine 27 (H3K27me3) induced by chilling specifically in DAM1 and DAM5, a 21-nt sRNA in DAM3 and a ncRNA induced in DAM4. Such induction is inversely correlated with downregulation of their cognate DAMs. We also show that the six DAMs were hypermethylated, associating with the production of 24-nt sRNAs. Hence, the chilling-responsive dynamic of the different epigenetic elements and their interactions likely define distinct expression abundance and downregulation pattern of each DAM. We further show that the expression of the five DAMs remains steadily unchanged or continuously downregulated at the ensuing warm temperature after chilling, and this state of regulation correlates with robust increase of sRNA expression, H3K27me3 and CHH methylation, which is particularly pronounced in DAM4. Such robust increase of repressive epigenetic marks may irreversibly reinforce the chilling-imposed repression of DAMs to ensure flower-developmental programming free from any residual DAM inhibition. Taken together, we reveal novel information about genetic and epigenetic regulation of the DAM cluster in peach, which will be of fundamental significance in understanding of the regulatory mechanisms underlying chilling requirement and dormancy release, and of practical application for improvement of plasticity of flower time and bud break in fruit trees to adapt changing climates.
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DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
C. S. Candice;J. Melissa;L. Clare;Dean Caroline;S. Elizabeth;Peacock W. James
通讯作者：
C. S. Candice;J. Melissa;L. Clare;Dean Caroline;S. Elizabeth;Peacock W. James
影响因子：
11.6
作者：
Liu, Yongxiu;Koornneef, Maarten;Soppe, Wim J. J.
通讯作者：
Soppe, Wim J. J.
影响因子：
--
作者：
Molly F. Thomas;K. Ansel
通讯作者：
Molly F. Thomas;K. Ansel
影响因子：
1.9
作者：
Arora, R;Rowland, LJ;Tanino, K
通讯作者：
Tanino, K
影响因子：
2.4
作者：
de la Fuente, Lorena;Conesa, Ana;Rios, Gabino
通讯作者：
Rios, Gabino