Development of a quartz crystal microbalance biosensor with aptamers as bio-recognition element.
Development of a quartz crystal microbalance biosensor with aptamers as bio-recognition element.
复制标题
以适体为生物识别元件的石英晶体微天平生物传感器的研制
DOI：
10.3390/s100605859
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Fu W
中科院分区：
文献类型：
--
作者：
Yao C;Zhu T;Qi Y;Zhao Y;Xia H;Fu W
任何生物传感器开发项目的最终目标都是将其用于实际样品检测。最近，人们对以适体作为生物识别元件的生物传感器产生了兴趣，但报道的例子都是针对标准品，而不是人血清。为了验证核酸适体生物传感器和抗体生物传感器在临床检测中的差异，比较了核酸适体和抗体石英晶体微天平（QCM）生物传感器检测人血清中免疫球蛋白E（IgE）的性能。将特异性针对IgE的适体（或抗体）固定在石英晶体的金表面上。测量了QCM的频移。与适体的线性范围（2.5-200 μ g/L）相比，抗体的线性范围（10-240 μ g/L），但在基于适体的生物传感器中可以观察到较低的检测限。两种生物传感器的重现性相当。在特异性和灵敏度方面，适体等同于或上级于抗体。此外，适体受体可以耐受配体结合和回收生物传感器后的重复仿射层再生，而几乎没有灵敏度的损失。当储存三周时，适配体包被的晶体的频率偏移均大于第一天响应的频率偏移的90%。
The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10–240 μg/L) compared to that of the aptamer (2.5–200 μg/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.
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影响因子：
7.4
作者：
Jiang, YX;Fang, XH;Bai, CL
通讯作者：
Bai, CL
影响因子：
6.2
作者：
Kim, Yeon Seok;Niazi, Javed H.;Gu, Man Bock
通讯作者：
Gu, Man Bock
影响因子：
7.4
作者：
Gokulrangan, G;Unruh, JR;Wilson, GS
通讯作者：
Wilson, GS
影响因子：
12.6
作者：
Tombelli, S;Mascini, M;Turner, APF
通讯作者：
Turner, APF
影响因子：
2.9
作者：
Wochner, Aniela;Menger, Marcus;Gloekler, Joern
通讯作者：
Gloekler, Joern