Cellular fatty acid uptake is acutely regulated by membrane-associated fatty acid-binding proteins.
Cellular fatty acid uptake is acutely regulated by membrane-associated fatty acid-binding proteins.
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细胞脂肪酸的摄取受到膜相关脂肪酸结合蛋白的敏锐调节。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Glatz
中科院分区：
文献类型：
--
作者：
J. Luiken;Arend Bonen;J. Glatz
细胞长链脂肪酸摄取被认为主要通过蛋白质介导的脂肪酸跨膜转运以及被动扩散摄取发生。据推测，膜蛋白的功能是从细胞外来源捕获脂肪酸，然后通过脂质双层的被动扩散发生跨膜转运。主要的膜相关蛋白是质膜脂肪酸结合蛋白（FABP（pm））和脂肪酸转位酶（FAT/CD 36）。它们的质膜含量与脂肪酸吸收率呈正相关。在心脏和骨骼肌的研究中，我们观察到FAT/CD 36是急性调节的，因为收缩和胰岛素都可以将FAT/CD 36从细胞内贮库转移到肌膜，从而增加脂肪酸摄取的速率。此外，从对肥胖Zucker大鼠（一种已建立的肥胖和胰岛素抵抗的啮齿动物模型）的研究中，已获得证据表明，在心脏、肌肉和脂肪组织中，FAT/CD 36从细胞内池永久性地重新定位到质膜，导致这种情况下脂肪酸摄取率增加。这些综合观察表明，蛋白质介导的脂肪酸摄取是细胞脂肪酸利用的关键步骤，并表明摄取过程的故障可能是胰岛素抵抗发病机制中的关键因素。
Cellular long-chain fatty acid uptake is believed to occur largely by protein-mediated transmembrane transport of fatty acids, and also by passive diffusional uptake. It is postulated that the membrane proteins function in trapping of fatty acids from extracellular sources, whereafter their transmembrane translocation occurs by passive diffusion through the lipid bilayer. The key membrane-associated proteins involved are plasma membrane fatty acid-binding protein (FABP(pm)) and fatty acid translocase (FAT/CD36). Their plasma membrane contents are positively correlated with rates of fatty acid uptake. In studies with heart and skeletal muscle we observed that FAT/CD36 is regulated acutely, in that both contraction and insulin can translocate FAT/CD36 from an intracellular depot to the sarcolemma, thereby increasing the rate of fatty acid uptake. In addition, from studies with obese Zucker rats, an established rodent model of obesity and insulin resistance, evidence has been obtained that in heart, muscle and adipose tissue FAT/CD36 is permanently relocated from an intracellular pool to the plasma membrane, resulting in increased fatty acid uptake rates in this condition. These combined observations indicate that protein-mediated fatty acid uptake is a key step in cellular fatty acid utilization, and suggest that malfunctioning of the uptake process could be a critical factor in the pathogenesis of insulin resistance.
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DOI：
10.1016/s0952-3278(99)80007-6
发表时间：
1999-05
期刊：
Prostaglandins, leukotrienes, and essential fatty acids
影响因子：
--
作者：
P. Watkins;J. Pevsner;S. Steinberg
通讯作者：
P. Watkins;J. Pevsner;S. Steinberg
影响因子：
4.3
作者：
P. Berk;D. Stump
通讯作者：
P. Berk;D. Stump
心脏巨囊泡中蛋白质介导的棕榈酸酯摄取和脂肪酸转运蛋白的表达。
影响因子：
6.5
作者：
Luiken,JJ;Turcotte,LP;Bonen,A
通讯作者：
Bonen,A
影响因子：
56.9
作者：
HALAAS, JL;GAJIWALA, KS;FRIEDMAN, JM
通讯作者：
FRIEDMAN, JM