Gene expression profiling of the paracrine effects of uterine natural killer cells on human endometrial epithelial cells.
Gene expression profiling of the paracrine effects of uterine natural killer cells on human endometrial epithelial cells.
复制标题
子宫自然杀伤细胞对人子宫内膜上皮细胞旁分泌作用的基因表达谱
DOI：
10.1155/2014/393707
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发表时间：
2014
影响因子：
2.8
通讯作者：
Jin Z
中科院分区：
文献类型：
--
作者：
Gong X;Chen Z;Liu Y;Lu Q;Jin Z
子宫内膜含有一群免疫细胞，这些细胞在着床和怀孕期间会发生变化。这些细胞大部分是子宫自然杀伤 (uNK) 细胞；然而，尚不清楚这些细胞如何与子宫内膜上皮细胞相互作用。因此，我们通过微阵列分析研究了 uNK 细胞分泌培养基对体外子宫内膜上皮细胞基因表达谱的旁分泌影响。我们的结果经 qRT-PCR 和蛋白质印迹验证，表明来自 uNK 细胞的可溶性因子改变了上皮细胞的基因表达谱。上调的基因包括白细胞介素 15 (IL-15) 和白细胞介素 15 受体 α (IL-15RA)，它们会形成刺激 uNK 细胞增殖的环。此外，血管内皮生长因子 C (VEGF-C) 和趋化因子（C-X-C 基序）配体 10 (CXCL-10) 在上皮细胞中也被确定上调，这表明 uNK 细胞与上皮细胞协同作用以支持着床和妊娠。此外，东方草药自古以来就被用来治疗不孕症；然而，我们未能发现紫丹饮可以调节这些子宫内膜旁分泌作用。
The endometrium contains a population of immune cells that undergo changes during implantation and pregnancy. The majority of these cells are uterine natural killer (uNK) cells; however, it is unclear how these cells interact with endometrial epithelial cells. Therefore, we investigated the paracrine effects of the uNK cell-secretion medium on the gene expression profile of endometrial epithelial cells in vitro through microarray analysis. Our results, which were verified by qRT-PCR and western blot, revealed that soluble factors from uNK cells alter the gene expression profiles of epithelial cells. The upregulated genes included interleukin-15 (IL-15) and interleukin-15 receptor alpha (IL-15RA), which result in a loop that stimulates uNK cell proliferation. In addition, vascular endothelial growth factor C (VEGF-C) and chemokine (C-X-C motif) ligand 10 (CXCL-10) were also determined to be upregulated in epithelial cells, which suggests that uNK cells work synergistically with epithelial cells to support implantation and pregnancy. In addition, oriental herbal medicines have been used to treat infertility since ancient times; however, we failed to find that Zi Dan Yin can regulate these endometrial paracrine effects.
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影响因子：
2.7
作者：
KING, A;WELLINGS, V;LOKE, YW
通讯作者：
LOKE, YW
DOI：
10.1084/jem.191.5.771
发表时间：
2000-03-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kennedy MK;Glaccum M;Brown SN;Butz EA;Viney JL;Embers M;Matsuki N;Charrier K;Sedger L;Willis CR;Brasel K;Morrissey PJ;Stocking K;Schuh JC;Joyce S;Peschon JJ
通讯作者：
Peschon JJ
影响因子：
6.1
作者：
Quenby, Siobhan;Nik, Helena;Bulmer, Judith
通讯作者：
Bulmer, Judith
影响因子：
6
作者：
Smith, Samantha D.;Dunk, Caroline E.;Jones, Rebecca L.
通讯作者：
Jones, Rebecca L.
影响因子：
5.5
作者：
Lash, Gendie E.;Schiessl, Barbara;Bulmer, Judith N.
通讯作者：
Bulmer, Judith N.