Mutations that improve the ant promoter of Salmonella phage P22.
Mutations that improve the ant promoter of Salmonella phage P22.
复制标题
改进沙门氏菌噬菌体 P22 的蚂蚁启动子的突变。
作者：
D. Graña;P. Youderian;M. Susskind
通过对四个严重的启动子下调突变的假逆转，分离到了增加噬菌体P22抗阻遏子基因启动子活性的突变。这些假复原体的序列变化包括单碱基对替换、单碱基对缺失、串联双碱基对缺失和多位点突变。单碱基对替换将非共识碱基对改变为位于-14和-8位置的共识碱基对。其他突变支持这样的观点，即保守的-35和-10六聚体之间的间隔区的长度是启动子强度的重要决定因素。间隔区中一个或两个碱基对的缺失显然通过将其从19个碱基对的间距移动到18或17个碱基对的间距来激活替代的-10六角体。
Mutations that increase the activity of the promoter for the antirepressor gene of phage P22 were isolated by pseudoreversion of four severe promoter-down mutations. The sequence changes in these pseudorevertants include single base pair substitutions, single base pair deletions, tandem double base pair deletions and multisite mutations. The single base pair substitutions change nonconsensus base pairs to consensus base pairs at positions -14 and -8. The other mutations provide support for the idea that the length of the spacer region between the conserved -35 and -10 hexamers is an important determinant of promoter strength. Deletions of one or two base pairs in the spacer region apparently activate an alternate -10 hexamer by shifting it from a spacing of 19 base pairs to a spacing of 18 or 17 base pairs, respectively.
噬菌体 P22 的 ImmI 免疫区的一级结构。
影响因子：
5.6
作者：
Sauer,RT;Krovatin,W;DeAnda,J;Youderian,P;Susskind,MM
通讯作者：
Susskind,MM
噬菌体 P22 的一个新基因，调节抗阻抑剂的合成。
影响因子：
5.6
作者：
Susskind,MM
通讯作者：
Susskind,MM