The role of SK3 in progesterone-induced inhibition of human fallopian tubal contraction.
The role of SK3 in progesterone-induced inhibition of human fallopian tubal contraction.
复制标题
SK3在黄体酮诱导的人类输卵管收缩抑制中的作用
DOI：
10.1186/s12958-022-00932-3
复制
发表时间：
2022-04-29
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
正常的输卵管运动活动对人类生殖至关重要，输卵管活动异常可能导致异位妊娠(EP)或不孕。黄体酮对输卵管收缩有抑制作用，但其机制尚不清楚。小电导钙激活钾通道3(Sk3)在血小板衍生生长因子受体α阳性(PDGFRα+)细胞中大量表达，并被认为在血管松弛中起重要作用。本研究旨在探讨SK3在人输卵管中的表达及其在孕酮抑制输卵管收缩中的作用。我们收集了因EP而行输卵管切除术的患者(EP组)和其他良性妇科疾病患者(非EP组)的输卵管标本。采用实时定量聚合酶链式反应、免疫印迹、免疫细胞化学、免疫组织化学等方法检测SK3的表达。采用等长张力实验研究SK3在孕酮抑制输卵管收缩中的作用。EP组人输卵管收缩的基线波幅和收缩频率均低于非EP组，差异有统计学意义。非EP组输卵管不同部位SK3的表达水平显著高于EP组。孕酮对两组大鼠输卵管收缩均有抑制作用，主要抑制幅度，可能与SK3等钙激活的K+通道有关。在人输卵管组织中检测到表达SK3的PDGFFRα(+)细胞。EP组SK3表达较低，SK3参与了孕酮抑制人输卵管收缩的作用。网上版载有补充材料，可在10.1186/s12958-022-00932-3查阅。
Normal motor activity of the fallopian tube is critical for human reproduction, and abnormal tubal activity may lead to ectopic pregnancy (EP) or infertility. Progesterone has an inhibitory effect on tubal contraction; however, the underlying mechanisms remain unclear. Small-conductance calcium-activated K+ channel 3 (SK3) is abundantly expressed in platelet-derived growth factor receptor α positive (PDGFRα+) cells and was reported to be important for the relaxation of smooth muscle. The present study aims to explore the expression of SK3 in the human fallopian tube and its role in progesterone-induced inhibition of tubal contraction. We collected specimens of fallopian tubes from patients treated by salpingectomy for EP (EP group) and other benign gynecological diseases (Non-EP group). The expression of SK3 was detected by quantitative real-time polymerase chain reaction, western blot, immunocytochemistry, and immunohistochemistry analyses. Isometric tension experiments were performed to investigate the role of SK3 in progesterone-induced inhibition of tubal contraction. The baseline amplitude and frequency of human fallopian tube contraction were both statistically lower in the EP group compared with the non-EP group. The expression levels of SK3 in different portions of fallopian tubes from the non-EP group were significantly higher than in those from the EP group. Progesterone had an inhibitory effect on tubal contraction, mainly on the amplitude, in both groups, and SK3 as well as other calcium-activated K+ channels may be involved. SK3-expressing PDGFRα (+) cells were detected in the human fallopian tube. The expression of SK3 is lower in the EP group, and SK3 is involved in the progesterone-induced inhibition of human fallopian tube contraction. The online version contains supplementary material available at 10.1186/s12958-022-00932-3.
登录
查看更多内容
DOI：
10.1186/s12958-015-0038-8
发表时间：
2015-05-13
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Bylander A;Gunnarsson L;Shao R;Billig H;Larsson DG
通讯作者：
Larsson DG
影响因子：
4.8
作者：
Milkau, Malte;Kohler, Ralf;de Wit, Cor
通讯作者：
de Wit, Cor
影响因子：
--
作者：
Dudem, Srikanth;Sergeant, Gerard P;Hollywood, Mark A
通讯作者：
Hollywood, Mark A
影响因子：
7.2
作者：
通讯作者：
--
影响因子：
6.1
作者：
Li, Hang Wun Raymond;Liao, Su-Bin;Ho, Pak-Chung
通讯作者：
Ho, Pak-Chung