Progesterone-mediated effects on gene expression and oocyte-cumulus complex transport in the mouse fallopian tube.
Progesterone-mediated effects on gene expression and oocyte-cumulus complex transport in the mouse fallopian tube.
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DOI：
10.1186/s12958-015-0038-8
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发表时间：
2015-05-13
期刊：
影响因子：
--
通讯作者：
Larsson DG
中科院分区：
文献类型：
--
作者：
Bylander A;Gunnarsson L;Shao R;Billig H;Larsson DG
输卵管将配子运送到受精点，并在最佳着床时间将胚胎送到子宫。黄体酮和经典黄体酮受体参与调节输卵管纤毛跳动和肌肉收缩，可能通过基因组和非基因组作用。为了提供更多潜在机制的细节，我们使用微阵列和/或定量PCR研究了孕酮在孕酮治疗后20分钟、2小时和8小时对小鼠输卵管基因表达的影响。与此同时，在注射孕激素受体拮抗剂Org 31710或CDB2194的排卵小鼠中，研究了卵母细胞积云复合体运输。微阵列分析在黄体酮治疗20分钟后未发现任何明显受调控的基因，这与黄体酮控制纤毛跳动的非基因组作用一致。2 h后，有11个基因表达上调。2 h和8 h的定量PCR分析显示，黄体酮一致上调内皮素1，下调其受体内皮素受体a。我们还证实，在排卵前使用孕酮受体拮抗剂可加速卵母细胞丘复合体的运输。本研究首次发现黄体酮调节输卵管内内皮素1和内皮素受体A的表达。结合先前关于内皮素对输卵管肌肉收缩影响的研究，本研究结果表明，内皮素是孕激素控制的输卵管肌肉收缩和最终配子运输的中介。本文的在线版本（doi:10.1186/s12958-015-0038-8）包含补充材料，仅供授权用户使用。
The fallopian tube transports the gametes to the fertilization site and delivers the embryo to the uterus at the optimal time for implantation. Progesterone and the classical progesterone receptor are involved in regulating both tubal ciliary beating and muscular contractions, likely via both genomic and non-genomic actions. To provide more details of the underlying mechanisms, we investigated the effect of progesterone on gene expression in mice fallopian tubes in vitro at 20 min, 2 h and 8 h post progesterone treatment using microarray and/or quantitative PCR. In parallel, oocyte cumulus complex transport was investigated in ovulating mice that were injected with one of the progesterone receptor antagonists, Org 31710 or CDB2194. Microarray analyses did not reveal any apparently regulated genes 20 min after progesterone treatment, consistent with the proposed non-genomic action of progesterone controlling ciliary beating. After 2 h, 11 genes were identified as up-regulated. Analyses using quantitative PCR at 2 h and 8 h showed a consistent up-regulation of endothelin1 and a down-regulation of its receptor Endothelin receptor A by progesterone. We also confirmed that treatment with progesterone receptor antagonists before ovulation accelerates the transport of the oocyte cumulus complex. This is the first study showing that progesterone regulates the expression of endothelin1 and endothelin receptor A in the fallopian tube. Together with previous studies of the effects of endothelin on muscular contractions in the fallopian tube, the results from this study suggest that endothelin is a mediator of the progesterone-controlled effects on muscular contraction and eventually gamete transport in the fallopian tube. The online version of this article (doi:10.1186/s12958-015-0038-8) contains supplementary material, which is available to authorized users.
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