The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes.
The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes.
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DOI：
10.1038/ncomms2589
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发表时间：
2013
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
负链RNA病毒的核蛋白形成负责病毒转录和复制的核糖核蛋白复合物的主要成分。然而，核蛋白在病毒RNA转录和复制中的确切作用尚不清楚。在这里，我们表明，甲型流感病毒的核蛋白是完全的复制和转录短病毒RNA样模板在体内，这表明核蛋白代表的病毒RNA聚合酶的延伸因子。我们还发现，在全长病毒基因复制过程中，核蛋白向新生核糖核蛋白复合物的募集是通过“尾环优先”方向的核蛋白-核蛋白同源寡聚化介导的，并且不依赖于RNA结合。这项工作表明，核蛋白不调节启动和终止的转录和复制的病毒聚合酶在体内，并提供了新的机制的见解组装和调节病毒核糖核蛋白复合物。
The nucleoprotein of negative strand RNA viruses forms a major component of the ribonucleoprotein complex that is responsible for viral transcription and replication. However, the precise role of nucleoprotein in viral RNA transcription and replication is not clear. Here we show that nucleoprotein of influenza A virus is entirely dispensable for replication and transcription of short viral RNA-like templates in vivo, suggesting that nucleoprotein represents an elongation factor for the viral RNA polymerase. We also find that the recruitment of nucleoprotein to nascent ribonucleoprotein complexes during replication of full length viral genes is mediated through nucleoprotein-nucleoprotein homo-oligomerisation in a “tail loop-first” orientation and is independent of RNA binding. This work demonstrates that nucleoprotein does not regulate the initiation and termination of transcription and replication by the viral polymerase in vivo and provides new mechanistic insights into the assembly and regulation of viral ribonucleoprotein complexes.
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