Single-Cell RNA Sequencing Reveals the Cellular Landscape of Longissimus Dorsi in a Newborn Suhuai Pig.
Single-Cell RNA Sequencing Reveals the Cellular Landscape of Longissimus Dorsi in a Newborn Suhuai Pig.
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单细胞RNA测序揭示新生苏淮猪背最长肌的细胞景观。
DOI：
10.3390/ijms25021204
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发表时间：
2024-01-18
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
单细胞RNA测序（scRNA-seq）技术的引入刺激了组织细胞组成分析的进一步发展。猪的背最长肌是研究猪肉品质的主要骨骼肌。然而，猪LD的单细胞谱仍处于起步阶段。在这项研究中，我们以单细胞分辨率分析了新生苏怀猪LD中16，018个细胞的转录组。随后，我们构建了LD的细胞图谱，鉴定了11个不同的细胞群，包括内皮细胞（24.39%），肌管（18.82%），成纤维脂肪祖细胞（FAPs，18.11%），卫星细胞（16.74%），成肌细胞（3.99%）、肌细胞（5.74%）、许旺细胞（3.81%）、平滑肌细胞（3.22%）、树突状细胞（2.99%）、周细胞（1.86%）和中性粒细胞（0.33%）。CellChat通过评估不同细胞类型中受体-配体对的基因表达来推断细胞-细胞相互作用。结果表明，FAP和周细胞是LD的主要信号贡献者。此外，我们描绘了生肌细胞的发育轨迹，并检查了在分化的各个阶段中各种标记基因和分子事件的表达的改变。此外，FAPs根据其生物学功能可分为NR 2F 2-FAPs、LPL-FAPs和TNMD-FAPs三个亚群，提示FAPs可能与肌腱细胞的分化有关。本研究构建了新生苏淮猪LD的细胞图谱和细胞通讯网络，分析了肌源性细胞的发育轨迹和FAPs亚群细胞的异质性。这增强了我们对猪骨骼肌发育和肉质控制所涉及的分子特征的理解。
The introduction of single-cell RNA sequencing (scRNA-seq) technology has spurred additional advancements in analyzing the cellular composition of tissues. The longissimus dorsi (LD) in pigs serves as the primary skeletal muscle for studying meat quality in the pig industry. However, the single-cell profile of porcine LD is still in its infancy stage. In this study, we profiled the transcriptomes of 16,018 cells in the LD of a newborn Suhuai pig at single-cell resolution. Subsequently, we constructed a cellular atlas of the LD, identifying 11 distinct cell populations, including endothelial cells (24.39%), myotubes (18.82%), fibro-adipogenic progenitors (FAPs, 18.11%), satellite cells (16.74%), myoblasts (3.99%), myocytes (5.74%), Schwann cells (3.81%), smooth muscle cells (3.22%), dendritic cells (2.99%), pericytes (1.86%), and neutrophils (0.33%). CellChat was employed to deduce the cell–cell interactions by evaluating the gene expression of receptor–ligand pairs across different cell types. The results show that FAPs and pericytes are the primary signal contributors in LD. In addition, we delineated the developmental trajectory of myogenic cells and examined alterations in the expression of various marker genes and molecular events throughout various stages of differentiation. Moreover, we found that FAPs can be divided into three subclusters (NR2F2-FAPs, LPL-FAPs, and TNMD-FAPs) according to their biological functions, suggesting that the FAPs could be associated with the differentiation of tendon cell. Taken together, we constructed the cellular atlas and cell communication network in LD of a newborn Suhuai pig, and analyzed the developmental trajectory of myogenic cells and the heterogeneity of FAPs subpopulation cells. This enhances our comprehension of the molecular features involved in skeletal muscle development and the meat quality control in pigs.
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