MYBPC2 and MYL1 as Significant Gene Markers for Rhabdomyosarcoma.
MYBPC2 and MYL1 as Significant Gene Markers for Rhabdomyosarcoma.
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DOI：
10.1177/1533033820979669
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发表时间：
2021-01
影响因子：
2.8
通讯作者：
Wang DL
中科院分区：
文献类型：
--
作者：
Chen Z;Li XY;Guo P;Wang DL
横纹肌肉瘤是儿童最常见的软组织肿瘤。横纹肌肉瘤常因肿瘤受压而引起疼痛和出血，且容易发生早期转移和复发，严重影响治疗效果和长期预后。高达 37.7% 的横纹肌肉瘤可能发生转移。因此，必须探索横纹肌肉瘤的分子机制，以确定其早期诊断和特异性治疗的有效靶点。下载了 18 个横纹肌肉瘤组织样本和 6 个健康骨骼肌样本的数据集。 GEO2R 鉴定了横纹肌肉瘤和健康组织样本之间的差异表达基因。进行了京都基因和基因组百科全书和基因本体通路富集分析。构建了蛋白质-蛋白质相互作用网络，并鉴定了枢纽基因。进行了 hub 基因的表达和存活分析。此外，还招募了 30 名横纹肌肉瘤患者，并收集了总体生存信息和样本。进行逆转录定量实时聚合酶链反应测定来验证横纹肌肉瘤肿瘤组织中MYBPC2和MYL1的表达。根据我们的临床数据，使用 Kaplan-Meier 方法来探索总生存期。横纹肌肉瘤肿瘤组织中共有 164 个基因上调，394 个基因下调。基因本体分析表明，变异主要集中在细胞周期、肌肉收缩、肌肉系统过程、细胞骨架、核苷酸结合和细胞骨架蛋白结合方面。蛋白质-蛋白质相互作用网络显示 3274 个边，构建了 441 个节点。鉴定出十个枢纽基因；其中，MYBPC2 和 MYL1 在横纹肌肉瘤中显着上调。与健康组相比，MYBPC2和MYL1高表达的横纹肌肉瘤患者的总生存率显着较差。我们发现横纹肌肉瘤和健康组织样本之间存在差异表达的基因。 MYBPC2和MYL1可能参与横纹肌肉瘤的发病机制，因此值得进一步探索。
Rhabdomyosarcoma is the most common soft tissue tumor in children. Rhabdomyosarcoma commonly results in pain and bleeding caused by tumor compression and is prone to early metastasis and recurrence, which can seriously affect the therapeutic outcomes and long-term prognosis. Up to 37.7% of rhabdomyosarcomas may metastasize. Therefore, the molecular mechanisms underlying rhabdomyosarcoma must be explored to identify an effective target for its early diagnosis and specific treatment. A dataset of 18 rhabdomyosarcoma tissue samples and 6 healthy skeletal muscle samples was downloaded. Differentially expressed genes between rhabdomyosarcoma and healthy tissue samples were identified by GEO2R. Kyoto Encyclopedia of Genes and Genomes and gene ontology pathway enrichment analyses were performed. A protein–protein interaction network was constructed, and hub genes were identified. Expression and survival analyses of hub genes were performed. Additionally, 30 patients with rhabdomyosarcoma were recruited, and overall survival information and samples were collected. Reverse transcription quantitative real-time polymerase chain reaction assays were performed to verify the expression of MYBPC2 and MYL1 in rhabdomyosarcoma tumor tissues. The Kaplan–Meier method was used to explore overall survival based on our clinical data. In total, 164 genes were up-regulated and 394 were down-regulated in rhabdomyosarcoma tumor tissues. Gene ontology analysis revealed that variations were predominantly enriched in the cell cycle, muscle contraction, muscle system processes, cytoskeleton, nucleotide binding, and cytoskeletal protein binding. The protein–protein interaction network revealed 3274 edges, and 441 nodes were constructed. Ten hub genes were identified; of these, MYBPC2 and MYL1 were significantly up-regulated in rhabdomyosarcoma. Compared with the healthy group, patients with rhabdomyosarcoma exhibiting high expression of MYBPC2 and MYL1 exhibited significantly worse overall survival. We found differentially expressed genes between rhabdomyosarcoma and healthy tissue samples. MYBPC2 and MYL1 may be involved in the pathogenesis of rhabdomyosarcoma and therefore deserve further exploration.
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DOI：
10.1158/1078-0432.ccr-19-2631
发表时间：
2020-03-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Casey DL;Wexler LH;Pitter KL;Samstein RM;Slotkin EK;Wolden SL
通讯作者：
Wolden SL
影响因子：
15.9
作者：
Bayram, Yavuz;Karaca, Ender;Lupski, James R.
通讯作者：
Lupski, James R.
影响因子：
45.3
作者：
Mascarenhas, Leo;Chi, Yueh-Yun;Hawkins, Douglas S.
通讯作者：
Hawkins, Douglas S.
影响因子：
1.7
作者：
Dasgupta, Roshni;Fuchs, Joerg;Rodeberg, David
通讯作者：
Rodeberg, David
CDK1 和 CCNB1 作为横纹肌肉瘤的潜在诊断标志物：生物信息学分析后的验证
影响因子：
2.7
作者：
Li, Qianru;Zhang, Liang;Li, Feng
通讯作者：
Li, Feng