Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks
Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks
复制标题
非结构化 mRNA 形成多价 RNA-RNA 相互作用以生成 TIS 颗粒网络
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
C. Mayr
中科院分区：
文献类型：
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作者：
Weirui Ma;Gang Zhen;W. Xie;C. Mayr
TIS颗粒网络是一种组成型表达的无膜细胞器，其浓缩具有富含AU的元件的mRNA，并与蛋白质合成的主要位点粗面内质网相互作用。大多数已知的生物分子凝聚物是球状的，但TIS颗粒具有网状形态。通过体内和体外重建实验，我们发现这种形状是由广泛的分子间RNA-RNA相互作用产生的。它们主要是由在其3′ UTR中具有大的非结构化区域的mRNA完成的，我们称之为固有无序区（IDR）。由于富含AU的RNA是抑制蛋白质聚集的有效伴侣，并且在具有IDR的mRNA中过度表达，因此我们的数据表明TIS颗粒浓缩有助于蛋白质折叠的mRNA。此外，TIS颗粒网络中翻译mRNA的接近性可能使共翻译蛋白质复合物形成成为可能。
The TIS granule network is a constitutively expressed membraneless organelle that concentrates mRNAs with AU-rich elements and interacts with the major site of protein synthesis, the rough endoplasmic reticulum. Most known biomolecular condensates are sphere-like, but TIS granules have a mesh-like morphology. Through in vivo and in vitro reconstitution experiments we discovered that this shape is generated by extensive intermolecular RNA-RNA interactions. They are mostly accomplished by mRNAs with large unstructured regions in their 3′UTRs that we call intrinsically disordered regions (IDRs). As AU-rich RNA is a potent chaperone that suppresses protein aggregation and is overrepresented in mRNAs with IDRs, our data suggests that TIS granules concentrate mRNAs that assist protein folding. In addition, the proximity of translating mRNAs in TIS granule networks may enable co-translational protein complex formation.
RNA控制Polyq蛋白相变。
影响因子：
16
作者：
Zhang H;Elbaum-Garfinkle S;Langdon EM;Taylor N;Occhipinti P;Bridges AA;Brangwynne CP;Gladfelter AS
通讯作者：
Gladfelter AS
影响因子：
4.5
作者：
Ding, Y;Chan, CY;Lawrence, CE
通讯作者：
Lawrence, CE