PAFAH1B3 Expression Is Correlated With Gastric Cancer Cell Proliferation and Immune Infiltration.
PAFAH1B3 Expression Is Correlated With Gastric Cancer Cell Proliferation and Immune Infiltration.
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PAFAH1B3表达与胃癌细胞增殖和免疫浸润相关
DOI：
10.3389/fonc.2021.591545
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发表时间：
2021
影响因子：
4.7
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Xie T;Guo X;Wu D;Li S;Lu Y;Wang X;Chen L
血小板激活因子乙酰水解酶 1b 催化亚基 3 (PAFAH1B3) 与多种人类疾病相关。然而，其在胃癌中的作用仍不确定。 PAFAH1B3 表达在癌症基因组图谱 (TCGA) 和基因型组织表达泛癌数据中进行了分析。使用 TCGA 临床生存数据评估 PAFAH1B3 表达与患者预后之间的关联。使用 clusterProfiler R 软件包对 PAFAH1B3 进行富集分析。此外，通过分析TCGA数据库评估PAFAH1B3表达与免疫细胞浸润之间的相关性。通过CCK8实验和集落形成实验评估PAFAH1B3对胃癌细胞增殖的影响。采用Transwell实验评价PAFAH1B3对胃癌细胞迁移的影响。采用蛋白质印迹法评估 PAFAH1B3 对胃癌细胞信号通路的作用。 PAFAH1B3在包括胃癌在内的多种肿瘤中高表达。 PAFAH1B3 高表达与涉及 DNA 复制、细胞周期和细胞周期检查点的增殖相关基因组显着相关。进一步分析表明，PAFAH1B3 高表达与 M1 巨噬细胞和 CD8 阳性 T 细胞浸润评分高相关。 PAFAH1B3 敲除抑制胃癌细胞的增殖、迁移和致癌信号的激活。我们的研究结果表明 PAFAH1B3 可能是胃癌的癌基因。
Platelet activating factor acetylhydrolase 1b catalytic subunit 3 (PAFAH1B3) is associated with a variety of human diseases. However, its function in gastric cancer remains uncertain. PAFAH1B3 expression was analyzed in The Cancer Genome Atlas (TCGA) and genotype-tissue expression pan-cancer data. The association between PAFAH1B3 expression and patient prognosis was evaluated using TCGA clinical survival data. Enrichment analysis of PAFAH1B3 was performed using the clusterProfiler R software package. Moreover, the correlation between PAFAH1B3 expression and immune cell infiltration were evaluated by analyzing TCGA database. CCK8 assay and colony-formation assay were performed to assess the effect of PAFAH1B3 on the proliferation of gastric cancer cells. Transwell assay was used to evaluate the impact of PAFAH1B3 on gastric cancer cell migration. Western blot was performed to evaluate the role of PAFAH1B3 on signaling pathways in gastric cancer cells. PAFAH1B3 was highly expressed in many types of tumors including gastric cancer. High PAFAH1B3 expression was significantly correlated with proliferation-related gene sets involved in DNA replication, the cell cycle, and cell cycle checkpoints. Further analysis showed that high PAFAH1B3 expression was associated with high M1 macrophage and CD8-positive T cell infiltration scores. PAFAH1B3 knockdown inhibited the proliferation, migration, and the activation of oncogenic signaling in gastric cancer cells. Our findings suggest that PAFAH1B3 may be an oncogene in gastric cancer.
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影响因子：
5.6
作者：
Reiss, Krzysztof;Del Valle, Luis;Lassak, Adam;Trojanek, Joanna
通讯作者：
Trojanek, Joanna
影响因子：
82.9
作者：
Binnewies M;Roberts EW;Kersten K;Chan V;Fearon DF;Merad M;Coussens LM;Gabrilovich DI;Ostrand-Rosenberg S;Hedrick CC;Vonderheide RH;Pittet MJ;Jain RK;Zou W;Howcroft TK;Woodhouse EC;Weinberg RA;Krummel MF
通讯作者：
Krummel MF
影响因子：
15.9
作者：
SATOH, K;IMAIZUMI, T;TAKAMATSU, M
通讯作者：
TAKAMATSU, M
影响因子：
5.1
作者：
Wang, Zhu;Yang, Yang;Wang, Shan
通讯作者：
Wang, Shan
影响因子：
3.2
作者：
Hirahara, Noriyuki;Tajima, Yoshitsugu;Taniura, Takahito
通讯作者：
Taniura, Takahito