Specific marker expression and cell state of Schwann cells during culture in vitro.
Specific marker expression and cell state of Schwann cells during culture in vitro.
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雪旺细胞体外培养过程中的特异性标志物表达和细胞状态
DOI：
10.1371/journal.pone.0123278
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Shen Z
中科院分区：
文献类型：
--
作者：
Liu Z;Jin YQ;Chen L;Wang Y;Yang X;Cheng J;Wu W;Qi Z;Shen Z
动物体内的雪旺细胞（SC）存在于不同的发育阶段或创伤修复阶段，主要通过SC特异性标志物的表达来区分。目前还没有研究确定SC在体外培养条件下的状态，SC中表达的特异性标志物也不清楚。本研究采用酶消化法分离新生小鼠坐骨神经干细胞，检测S100、p75 NTR、Sox 10、Sox 2、GAP 43、NCAM、Krox 20、Oct 6、MBP和MPZ等10个标记物在体外培养小鼠干细胞中的RNA和蛋白表达，并推测其与体内干细胞分期的关系。我们通过免疫荧光、Western Blot和实时定量RT-PCR检测SC特异性标志物的RNA和蛋白水平。结果显示，在所有培养的早期SC中，S100、p75 NTR、GAP 43、NCAM、Krox 20、Oct 6、MBP和MPZ等标记物均未检测到表达。然而，在所有SC中均可检测到转录因子Sox 10和Sox 2的表达。8 d后，除GAP 43和Oct 6外，其余标记的阳性表达率均接近100%，说明Sox 10是鉴定SC的必要标记，而S100不可靠。体外培养的干细胞表达Sox 2、P75 NTR、NCAM、GAP 43、Oct 6和MPZ，表明它们与神经损伤后体内未分化的iSC或去分化的iSC相似。
Schwann cells (SCs) in animals exist in different developmental stages or wound repair phases, distinguished mainly by the expression of SC-specific markers. No study has yet determined SC state under in vitro culture conditions, and the specific markers expressed in SC are obscure as well. In this study, we harvested sciatic nerves from newborn mice and isolated SCs by an enzyme-digestion method, then we examined the expression profiles of ten markers (S100, p75NTR, Sox10, Sox2, GAP43, NCAM, Krox20, Oct6, MBP, and MPZ) at both the RNA and protein levels in in vitro mouse SCs and speculated their relation with in vivo SC stages. We assayed RNA and protein levels of SC specific markers by immunofluorescence, Western Blot, and real-time quantitative RT-PCR. The results show that the expression of most markers (S100, p75NTR, GAP43, NCAM, Krox20, Oct6, MBP and MPZ) was not detectable in all of early stage cultured SCs. The expression of transcription factors Sox10 and Sox2 was, however, detectable in all SCs. After 8 days, the positive expression rate of all markers except GAP43 and Oct6 was almost 100%.These results indicates Sox10 is a necessary marker for SC identification, while S100 is not reliable. SCs cultured in vitro express Sox2, P75NTR, NCAM, GAP43, Oct6, and MPZ, suggesting that they are similar to in vivo undifferentiated iSCs or dedifferentiated iSCs after nerve injury.
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DOI：
10.1083/jcb.200912142
发表时间：
2010-05-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Finzsch M;Schreiner S;Kichko T;Reeh P;Tamm ER;Bösl MR;Meijer D;Wegner M
通讯作者：
Wegner M
在沃勒式变性过程中，瞬时溶酶体激活对于髓鞘的Schwann细胞中的p75神经生长因子受体表达至关重要。
影响因子：
1.1
作者：
Jung J;Cai W;Jang SY;Shin YK;Suh DJ;Kim JK;Park HT
通讯作者：
Park HT
影响因子：
9.1
作者：
Heinen, Andre;Lehmann, Helmar C.;Kuery, Patrick
通讯作者：
Kuery, Patrick
影响因子：
3.4
作者：
Dezawa, M;Takahashi, I;Sawada, H
通讯作者：
Sawada, H
影响因子：
10.5
作者：
Avilion, AA;Nicolis, SK;Lovell-Badge, R
通讯作者：
Lovell-Badge, R