Development and validation of InDel markers for identification of QTL underlying flowering time in soybean
Development and validation of InDel markers for identification of QTL underlying flowering time in soybean
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用于鉴定大豆开花时间的 QTL 的 InDel 标记的开发和验证
DOI：
10.1016/j.cj.2017.08.001
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发表时间：
2017-09
期刊：
影响因子：
6.6
通讯作者：
Kong Fanjiang
中科院分区：
文献类型：
--
作者：
Wang Jialin;Kong Lingping;Yu Kanchao;Zhang Fengge;Shi Xinyi;Wang Yanping;Nan Haiyang;Zhao Xiaohui;Lu Sijia;Cao Dong;Li Xiaoming;Fang Chao;Wang Feifei;Su Tong;Li Shichen;Yuan Xiaohui;Liu Baohui;Kong Fanjiang
Soybean [Glycinemax (L.) Merrill] is a major plant source of protein and oil. An accurate and well-saturated molecular linkage map is a prerequisite for forward genetic studies of gene function and for modern breeding for many useful agronomic traits. Next-generation sequence data available in public databases provides valuable information and offers new insights for rapid and efficient development of molecular markers. In this study, we attempted to show the feasibility and facility of using genomic resequencing data as raw material for identifying putative InDel markers. First, we identified 17,613 InDel sites among 56 soybean accessions and obtained 12,619 primer pairs. Second, we constructed a genetic map with a random subset of 2841 primer pairs and aligned 300 polymorphic markers with the 20 consensus linkage groups (LG). The total genetic distance was 2347.3 cM and the number of mapped markers per LG ranged from 10 to 23 with an average of 15 markers. The largest and smallest genetic distances between adjacent markers were 52.3 cM and 0.1 cM, respectively. Finally, we validated the genetic map constructed by newly developed InDel markers by QTL analysis of days to flowering (DTF) under different environments. One major QTL (qDTF4) and four minor QTL (qDTF20,qDTF13,qDTF12, andqDTF11) on 5 LGs were detected. These results demonstrate the utility of the InDel markers developed in this work for map-based cloning and molecular breeding in soybean.
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影响因子：
5.6
作者：
Sohn HB;Kim SJ;Hwang TY;Park HM;Lee YY;Markkandan K;Lee D;Lee S;Hong SY;Song YH;Koo BC;Kim YH
通讯作者：
Kim YH
影响因子：
4.4
作者：
Yang H;Tao Y;Zheng Z;Li C;Sweetingham MW;Howieson JG
通讯作者：
Howieson JG
基于 InDel 的辣椒 (Capsicum annuum) 连锁图谱
DOI：
10.1007/s11032-015-0219-3
发表时间：
2015
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Li W;Cheng J;Wu Z;Qin C;Tan S;Tang X;Cui J;Zhang L;Hu K
通讯作者：
Hu K
影响因子：
3.1
作者：
Garcia-Lor, Andres;Luro, Francois;Ollitrault, Patrick
通讯作者：
Ollitrault, Patrick
影响因子：
3.1
作者：
H. Raman;R. Raman;Rachel H. Wood;P. Martin
通讯作者：
H. Raman;R. Raman;Rachel H. Wood;P. Martin