High-throughput proteomic profiling of the fish liver following bacterial infection.
High-throughput proteomic profiling of the fish liver following bacterial infection.
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DOI：
10.1186/s12864-018-5092-0
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发表时间：
2018-10-01
期刊：
影响因子：
4.4
通讯作者：
Macqueen DJ
中科院分区：
文献类型：
--
作者：
Causey DR;Pohl MAN;Stead DA;Martin SAM;Secombes CJ;Macqueen DJ
高通量蛋白质组学被用来确定鱼肝脏防御细菌感染的反应中的作用。这是使用虹鳟鱼（虹鳟鱼）模型感染杀鲑气单胞菌，呋喃酮的病原体。脊椎动物的肝脏在先天免疫、代谢和生长方面具有多方面的功能;我们假设该组织在支持宿主防御和促进有效免疫功能的代谢调节方面具有双重作用。虽然过去的研究已经报道了mRNA对A.尽管在鲑科鱼类中存在杀鲑素，但细菌感染对肝脏蛋白质组的影响在鱼类中仍然没有特征。虹鳟鱼注射A.杀鲑素或PBS（对照），48小时后提取肝脏，用于在混合四极杆-Orbitrap质谱仪上分析。无标记方法用于蛋白质丰度分析，其揭示了强烈的先天免疫应答沿着支持代谢和生长系统的平行重新布线的证据。针对单个高质量虹鳟参考基因组中注释的所有蛋白质（n = 71，293个RefSeq蛋白质），最初鉴定了3076个蛋白质，其中2433个蛋白质被保留用于质量过滤后的分析。在2433个蛋白质中，有109个蛋白质在A.杀鲑素挑战，包括许多上调的补体系统和急性期反应蛋白，以及具有可能支持代谢重新调整的推定功能的分子。我们还确定了新的扩张，补体系统由于基因和全基因组复制事件在鲑鱼的进化史，包括8个C3蛋白质显示丰度的差异变化。这项研究提供了第一个高通量的蛋白质组学检查鱼肝脏响应细菌的挑战，揭示了新的标志物的主机防御反应，并与先天免疫激活代谢重塑的证据。本文的在线版本（10.1186/s12864-018-5092-0）包含补充材料，可供授权用户使用。
High-throughput proteomics was used to determine the role of the fish liver in defense responses to bacterial infection. This was done using a rainbow trout (Oncorhynchus mykiss) model following infection with Aeromonas salmonicida, the causative agent of furunculosis. The vertebrate liver has multifaceted functions in innate immunity, metabolism, and growth; we hypothesize this tissue serves a dual role in supporting host defense in parallel to metabolic adjustments that promote effective immune function. While past studies have reported mRNA responses to A. salmonicida in salmonids, the impact of bacterial infection on the liver proteome remains uncharacterized in fish. Rainbow trout were injected with A. salmonicida or PBS (control) and liver extracted 48 h later for analysis on a hybrid quadrupole-Orbitrap mass spectrometer. A label-free method was used for protein abundance profiling, which revealed a strong innate immune response along with evidence to support parallel rewiring of metabolic and growth systems. 3076 proteins were initially identified against all proteins (n = 71,293 RefSeq proteins) annotated in a single high-quality rainbow trout reference genome, of which 2433 were maintained for analysis post-quality filtering. Among the 2433 proteins, 109 showed significant differential abundance following A. salmonicida challenge, including many upregulated complement system and acute phase response proteins, in addition to molecules with putative functions that may support metabolic re-adjustments. We also identified novel expansions in the complement system due to gene and whole genome duplication events in salmonid evolutionary history, including eight C3 proteins showing differential changes in abundance. This study provides the first high-throughput proteomic examination of the fish liver in response to bacterial challenge, revealing novel markers for the host defense response, and evidence of metabolic remodeling in conjunction with activation of innate immunity. The online version of this article (10.1186/s12864-018-5092-0) contains supplementary material, which is available to authorized users.
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