Aminopropyltransferases involved in polyamine biosynthesis localize preferentially in the nucleus of plant cells.
Aminopropyltransferases involved in polyamine biosynthesis localize preferentially in the nucleus of plant cells.
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DOI：
10.1371/journal.pone.0046907
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ferrando A
中科院分区：
文献类型：
--
作者：
Belda-Palazón B;Ruiz L;Martí E;Tárraga S;Tiburcio AF;Culiáñez F;Farràs R;Carrasco P;Ferrando A
植物氨丙基转移酶由一组转移脱羧S-腺苷甲硫氨酸衍生的氨丙基的酶组成亚精胺合酶（SPDS）使用腐胺作为氨基受体形成亚精胺，而精胺合成酶（SPMS）和热精胺合成酶（TSPMS）分别以精脒为受体合成精胺和热精胺异构体。在之前的工作中，尽管没有报道有关亚细胞定位的数据，但研究表明SPDS 1和SPDS 2都可以与SPMS发生物理相互作用。在这里，我们研究了这些酶和它们的蛋白质二聚体复合物的亚细胞定位与基于网关的双分子荧光互补（BiFC）的二元载体。此外，我们的特点是通过凝胶过滤色谱与体外组装的重组酶和内源性植物蛋白提取物的酶复合物的分子量。我们的数据表明，氨丙基转移酶显示出双重亚细胞定位在细胞质和核富集的馏分，他们组装优选为二聚体。BiFC瞬时表达数据表明，氨丙基转移酶异二聚体复合物优先发生在细胞核内。
Plant aminopropyltransferases consist of a group of enzymes that transfer aminopropyl groups derived from decarboxylated S-adenosyl-methionine (dcAdoMet or dcSAM) to propylamine acceptors to produce polyamines, ubiquitous metabolites with positive charge at physiological pH. Spermidine synthase (SPDS) uses putrescine as amino acceptor to form spermidine, whereas spermine synthase (SPMS) and thermospermine synthase (TSPMS) use spermidine as acceptor to synthesize the isomers spermine and thermospermine respectively. In previous work it was shown that both SPDS1 and SPDS2 can physically interact with SPMS although no data concerning the subcellular localization was reported. Here we study the subcellular localization of these enzymes and their protein dimer complexes with gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vectors. In addition, we have characterized the molecular weight of the enzyme complexes by gel filtration chromatography with in vitro assembled recombinant enzymes and with endogenous plant protein extracts. Our data suggest that aminopropyltransferases display a dual subcellular localization both in the cytosol and nuclear enriched fractions, and they assemble preferably as dimers. The BiFC transient expression data suggest that aminopropyltransferase heterodimer complexes take place preferentially inside the nucleus.
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影响因子：
7.2
作者：
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影响因子：
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