Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis.
Decellularised extracellular matrix decorated PCL PolyHIPE scaffolds for enhanced cellular activity, integration and angiogenesis.
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DOI：
10.1039/d1bm01262b
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发表时间：
2021-10-26
影响因子：
6.6
通讯作者：
Claeyssens F
中科院分区：
文献类型：
--
作者：
Dikici S;Aldemir Dikici B;MacNeil S;Claeyssens F
伤口愈合涉及一系列复杂的事件，其中细胞-细胞和细胞-细胞外基质（ECM）相互作用起关键作用。创伤可以是简单的，例如上皮完整性的丧失，或更深和更复杂的，达到皮下组织，包括血管，肌肉和神经。伤口区域的快速新生血管形成对于伤口愈合至关重要，因为它在高度要求的增殖阶段和炎症细胞向伤口区域的迁移期间在供应氧气和营养物方面具有关键作用。避免延迟新生血管形成的一种方法是外源性使用促血管生成因子，其是昂贵的、高度剂量依赖性的，并且它们的递送需要非常好地控制的系统以避免渗漏的、高度可渗透的和出血的血管形成。在这项研究中，我们用成纤维细胞衍生的ECM修饰了基于聚己内酯（PCL）的聚合高内相乳液（PolyHIPE）支架，以评估体外成纤维细胞、内皮细胞和角质形成细胞的活性以及卵外鸡绒膜尿囊膜（CAM）中的血管生成（CAM）测定。我们的结果表明，在支架中包含ECM增加了三种类型的细胞的代谢活性，这些细胞在7天内的卵外CAM测定中在伤口愈合和刺激血管生成中起关键作用。在此，我们证明了成纤维细胞-ECM功能化PCL PolyHIPE支架似乎具有用作活性伤口敷料以促进血管生成和伤口愈合的巨大潜力。体外产生的细胞外基质（ECM）的去细胞化提供了刺激血管生成和伤口愈合的有效方式。
Wound healing involves a complex series of events where cell–cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity in vitro and angiogenesis in ex ovo chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in ex ovo CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing. Decellularisation of in vitro generated extracellular matrix (ECM) provides an effective way to stimulate angiogenesis and wound healing.
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影响因子：
4.6
作者：
Arruda Pereira, Rayssa Helena;Prado, Adilson Ribeiro;Nogueira, Breno Valentim
通讯作者：
Nogueira, Breno Valentim
影响因子：
5.5
作者：
Barbetta, A;Cameron, NR
通讯作者：
Cameron, NR
影响因子：
9.8
作者：
Bishop, E T;Bell, G T;Wheatley, D N
通讯作者：
Wheatley, D N
DOI：
10.1016/b978-0-12-812258-7.00005-8
发表时间：
2019-01-01
期刊：
STEM CELLS AND BIOMATERIALS FOR REGENERATIVE MEDICINE
影响因子：
--
作者：
Akbari-Birgani, Shiva;Birgani, Maryam Tahmasebi;Ansari, Hossein
通讯作者：
Ansari, Hossein
影响因子：
6
作者：
Can-Herrera, L. A.;Avila-Ortega, A.;Cervantes-Uc, J. M.
通讯作者：
Cervantes-Uc, J. M.