Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia
Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia
复制标题
哥伦比亚马铃薯黄脉病毒和马铃薯Y病毒对马铃薯作物的自然共感染
DOI：
10.15446/agron.colomb.v32n2.43968
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发表时间：
2014
影响因子：
2.7
通讯作者：
M. Guzmán
中科院分区：
文献类型：
--
作者：
Angela Villamil;W. Cuellar;M. Guzmán
马铃薯黄脉病毒（PYVV）是一种具有RNA三重基因组的Crinivirus，是自1950年以来在哥伦比亚报道的马铃薯黄脉病的病原体，产量减少高达50%。两种或多种病毒的共感染在自然界中很常见，并且可能与病毒积累和症状表达的差异有关。没有证据表明PYVV与其他病毒混合感染。在这项研究中，八个植物表现出黄化PYVV症状：四个马铃薯组Escherija（P）和四个组Andigena（A），收集在哥伦比亚的昆迪纳马卡，使用下一代测序（NGS）检测分离物中的混合感染。利用NGS技术对马铃薯Y病毒（PVY）全基因组（与N株相似）和马铃薯V病毒（PVV）部分基因组进行了检测，并通过RT-PCR技术进行了验证。在大样本中的初步田间筛选显示，PYVV和PVY共感染马铃薯植物，P组内的患病率为21%，A组内的患病率为23%。这是在哥伦比亚首次报告PYVV和马铃薯Y病毒合并感染，并使用NGS。考虑到马铃薯Y病毒增强症状的严重程度和/或产量减少的混合感染，我们的研究结果可能是相关的疾病诊断，育种计划和块茎认证。
The Potato yellow vein virus (PYVV), a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P) and four Group Andigena (A), were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS). The Potato virus Y (PVY) complete genome (similar to N strain) and the Potato virus V (PVV) partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and/or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.
影响因子：
3.8
作者：
Cuellar, Wilmer J.;De Souza, Joao;Kreuze, Jan F.
通讯作者：
Kreuze, Jan F.