The regulatory role of ferric uptake regulator (Fur) during anaerobic respiration of Shewanella piezotolerans WP3.
The regulatory role of ferric uptake regulator (Fur) during anaerobic respiration of Shewanella piezotolerans WP3.
复制标题
铁吸收调节剂（毛皮）在压电希瓦氏菌 WP3 无氧呼吸过程中的调节作用
DOI：
10.1371/journal.pone.0075588
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang FP
中科院分区：
文献类型：
--
作者：
Yang XW;He Y;Xu J;Xiao X;Wang FP
铁摄取调节剂(Fur)是一种控制细菌铁稳态的全球性调节剂。本研究构建了深海细菌希瓦氏杆菌WP3的毛皮缺失突变体。生理研究表明，该突变体在有氧条件下的生长速度仅略低于野生型(WT)，但在厌氧条件下，当提供不同的电子受体(EAs)时，会出现严重的生长缺陷。比较转录分析表明，Fur不仅参与了经典的铁稳态，而且还参与了无氧呼吸。FUR通过控制无氧电子传递、血红素生物合成系统和细胞色素c成熟系统对无氧呼吸的调节起着多效性作用。生化分析表明，在毛皮突变体中C型细胞色素的水平较低，这与转录图谱一致。转录分析和电泳迁移率改变分析表明，WP3中的Fur存在一个初级调控网络。这些结果表明，毛皮可能作为缺氧条件的传感器来触发和影响无氧呼吸系统。
Ferric uptake regulator (Fur) is a global regulator that controls bacterial iron homeostasis. In this study, a fur deletion mutant of the deep-sea bacterium Shewanella piezotolerans WP3 was constructed. Physiological studies revealed that the growth rate of this mutant under aerobic conditions was only slightly lower than that of wild type (WT), but severe growth defects were observed under anaerobic conditions when different electron acceptors (EAs) were provided. Comparative transcriptomic analysis demonstrated that Fur is involved not only in classical iron homeostasis but also in anaerobic respiration. Fur exerted pleiotropic effects on the regulation of anaerobic respiration by controlling anaerobic electron transport, the heme biosynthesis system, and the cytochrome c maturation system. Biochemical assays demonstrated that levels of c-type cytochromes were lower in the fur mutant, consistent with the transcriptional profiling. Transcriptomic analysis and electrophoretic mobility shift assays revealed a primary regulation network for Fur in WP3. These results suggest that Fur may act as a sensor for anoxic conditions to trigger and influence the anaerobic respiratory system.
登录
查看更多内容
影响因子：
4.4
作者：
Gao H;Wang X;Yang ZK;Palzkill T;Zhou J
通讯作者：
Zhou J
影响因子：
4.4
作者：
Bender, Kelly S.;Yen, Huei-Che Bill;Wall, Judy D.
通讯作者：
Wall, Judy D.
影响因子：
3.6
作者：
Gralnick, JA;Brown, CT;Newman, DK
通讯作者：
Newman, DK
DOI：
10.1073/pnas.85.6.1888
发表时间：
1988-03-01
影响因子：
11.1
作者：
IUCHI, S;LIN, ECC
通讯作者：
LIN, ECC
DOI：
10.1073/pnas.032066599
发表时间：
2002-04-02
影响因子：
11.1
作者：
Massé, E;Gottesman, S
通讯作者：
Gottesman, S