Deep UV microscopy of prostate cancer tissue
Deep UV microscopy of prostate cancer tissue
复制标题
前列腺癌组织的深紫外显微镜
DOI：
10.1117/12.2546200
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Robles, Francisco E.
中科院分区：
文献类型：
--
作者：
Soltani, Soheil;Ojaghi, Ashkan;Kludze, Atsutse;Robles, Francisco E.
正确诊断和分期前列腺癌仍然是一个重大的临床挑战。目前，护理标准包括病理学家对苏木精-伊红染色（HE）组织学切片的目视评估，以及基于前两种最常见模式指定Gleason评分。然而，这个过程是主观的，因此容易出错。此外，缺乏用于染色的标准方案使得染色组织的定量分析变得困难。因此，非常需要开发新的定量方法，其可以提供前列腺癌的侵袭性和阶段的稳健、客观和准确的信息。在这项工作中，我们试图解决这一挑战，使用多光谱深紫外显微镜的未染色的组织切片。这种方法由于其亚细胞空间分辨率和对许多内源性生物分子（包括核酸和蛋白质）的高灵敏度而产生对疾病的侵袭性和阶段的有价值的洞察。在我们的方法中，我们使用一个简单的和具有成本效益的宽视场成像配置，在多个波长范围从220 nm到450 nm的顺序照明。光谱特征与形态学一起使用主成分分析的几何表示和数学形态学的原理进行分析。我们的研究结果揭示了随着癌症变得更具侵袭性，组织中明显的形态学和分子学改变。在本报告中，我们将详细介绍设计的多光谱，深紫外显微镜;描述我们的定量图像分析;并显示初步结果。
Correctly diagnosing and staging prostate cancer continues to be a significant clinical challenge. Currently, the standard of care consists of a pathologist’s visual assessment of hematoxylin-and-eosin-stained (HE) histological sections, and designation of a Gleason score based on the top two most common patterns. However, this process is subjective and thus prone to error. Further, lack of standard protocols for staining, makes quantitative analysis of stained tissues difficult. Therefore, there is a significant need to develop new quantitative methods that can provide robust, objective, and accurate information of the aggressiveness and stage of prostate cancer. In this work, we seek to address this challenge using multi-spectral deep-UV microscopy of unstained tissue sections. This method yields valuable insight into the aggressiveness and stage of the disease due to its subcellular spatial resolution and high sensitivity to many endogenous biomolecules, including nucleic acid and proteins. In our approach we use a simple and cost effective wide-field imaging configuration with sequential illumination at multiple wavelengths ranging from 220 nm to 450 nm. Spectral signatures are analyzed in conjunction with the morphology using a geometrical representation of principal component analysis and principles of mathematical morphology. Our results reveal distinct morphological and molecular alterations in the tissue as cancer becomes more aggressive. In this presentation we will detail the design of the multispectral, deep UV microscope; describe our quantitative image analysis; and show preliminary results.
当代前列腺癌分级：对患者护理的影响
DOI：
10.1016/j.yuro.2013.06.014
发表时间：
2013
期刊：
Yearbook of Urology
影响因子：
--
作者：
G. Andriole
通讯作者：
G. Andriole
影响因子：
4.6
作者：
Ojaghi A;Fay ME;Lam WA;Robles FE
通讯作者：
Robles FE