Ultraviolet Hyperspectral Interferometric Microscopy.
Ultraviolet Hyperspectral Interferometric Microscopy.
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DOI:
10.1038/s41598-018-28208-0
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发表时间:
2018-07-02
影响因子:
4.6
通讯作者:
Robles FE
中科院分区:
文献类型:
--
作者:
Ojaghi A;Fay ME;Lam WA;Robles FE
Ultraviolet (UV) spectroscopy is a powerful tool for quantitative (bio)chemical analysis, but its application to molecular imaging and microscopy has been limited. Here we introduce ultraviolet hyperspectral interferometric (UHI) microscopy, which leverages coherent detection of optical fields to overcome significant challenges associated with UV spectroscopy when applied to molecular imaging. We demonstrate that this method enables quantitative spectral analysis of important endogenous biomolecules with subcellular spatial resolution and sensitivity to nanometer-scaled structures for label-free molecular imaging of live cells.
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