Effects of decreasing the frequency of gonadotropin-releasing hormone stimulation on gonadotropin secretion in gonadotropin-releasing hormone-deficient men and perifused rat pituitary cells.
Effects of decreasing the frequency of gonadotropin-releasing hormone stimulation on gonadotropin secretion in gonadotropin-releasing hormone-deficient men and perifused rat pituitary cells.
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减少促性腺激素释放激素刺激的频率对促性腺激素释放激素缺乏的男性和灌注的大鼠垂体细胞中促性腺激素分泌的影响。
DOI：
10.1172/jci113512
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Crowley,WF
中科院分区：
文献类型：
--
作者：
Finkelstein,JS;Badger,TM;O'Dea,LS;Spratt,DI;Crowley,WF
本文研究了降低促性腺激素释放激素(GnRH)搏动性刺激频率对单纯性GnRH缺乏症患者和(B)在无性激素的情况下雄性大鼠分散的垂体腺细胞在长期搏动性GnRH替代治疗中达到正常性激素水平的影响。在三组4名GnRH缺陷男性中，GnRH刺激频率每周减少一次，从(A)每2-3-4h(组I)，(B)每2-8h(组II)，或(C)每2-8h(组III)。每隔2、4、8h给药1次，I、II组促黄体生成素(L H)曲线下平均面积随刺激次数的减少而增加(P<0.025)，血清睾酮水平降低(P<0.035)。在组III中，尽管血清睾酮水平不变，但黄体生成素曲线下面积也增加(P<0.01)，从而证明垂体腺对低频率GnRH刺激的反应性增加与性激素激素环境的变化无关。当GnRH给药频率降至每8h给药一次时，分散的大鼠垂体细胞的黄体生成素曲线下面积也增加(P<0.05)，这表明即使在完全去掉性腺激素的情况下，垂体对低频率GnRH刺激的反应性仍然增强。随着促性腺激素释放激素刺激频率的降低，三组的nadir-Lh水平均下降(P<0.01)。相反，随着促性腺激素释放激素刺激频率的降低，平均峰值黄体生成素水平、黄体生成素上升速率和内源性黄体生成素衰减率均保持不变。最后，随着促性腺激素释放激素脉冲间隔的增加，平均促黄体生成素水平下降，而平均促卵泡激素水平稳定或下降。这些结果表明：(A)在体内和体外模型中，GnRH刺激频率较低时，垂体腺对GnRH的反应性增强；(B)垂体腺对GnRH反应性的改变与性腺激素分泌的变化无关；(C)在GnRH刺激频率较低时，垂体对GnRH的脉冲幅度和曲线下面积的增加是由于谷值的降低，而不是峰值。GnRH分泌频率减慢可能是控制垂体促性腺激素分泌的一个重要自变量。
The effects of decreasing the frequency of pulsatile gonadotropin-releasing hormone (GnRH) stimulation on pituitary responsiveness were studied in (a) men with isolated GnRH deficiency who had achieved normal sex steroid levels during prior long-term pulsatile GnRH replacement and (b) perifused dispersed pituitary cells from male rats in the absence of sex steroids. In three groups of four GnRH-deficient men, the frequency of GnRH stimulation was decreased at weekly intervals from (a) every 2-3-4 h (group I), (b) every 2-8 h without testosterone replacement (group II), or (c) every 2-8 h with testosterone replacement (group III). In three groups of three columns of perifused dispersed pituitary cells, pulses of GnRH were administered every 2, 4, or 8 h. In groups I and II, mean area under the luteinizing hormone (LH) curve increased (P less than 0.025) and serum testosterone levels fell (P less than 0.035) as the frequency of GnRH stimulation was decreased. In group III, the area under the LH curve also increased (P less than 0.01) although serum testosterone levels were constant, thereby demonstrating that the increase in pituitary responsiveness to slow frequencies of GnRH stimulation occurs independently of changes in the sex steroid hormonal milieu. The area under the LH curve also increased in the perifused dispersed rat pituitary cells when the frequency of GnRH administration was decreased to every 8 h (P less than 0.05), thus demonstrating that the enhanced pituitary responsiveness to slow frequencies of GnRH stimulation is maintained even in the complete absence of gonadal steroids. Nadir LH levels fell in all three groups (P less than 0.01) as the frequency of GnRH stimulation was decreased. In contrast, mean peak LH levels, the rate of LH rise, and the rate of endogenous LH decay were constant as the frequency of GnRH stimulation was decreased. Finally, as the GnRH interpulse interval increased, mean LH levels fell, and mean follicle-stimulating hormone levels were stable or fell. These results indicate that (a) pituitary responsiveness to GnRH increases at slower frequencies of GnRH stimulation in models both in vivo and in vitro, (b) these changes in pituitary responsiveness occur independently of changes in gonadal steroid secretion, and (c) the increases in LH pulse amplitude and area under the curve at slow frequencies of GnRH stimulation are due to decreases in nadir, but not peak, LH levels. Slowing of the frequency of GnRH secretion may be an important independent variable in the control of pituitary gonadotropin secretion.Images
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影响因子：
2.7
作者：
P. Rao;P. H. Moore
通讯作者：
P. H. Moore
促性腺激素释放激素脉冲特征对灌注羊垂体细胞黄体生成激素释放动力学的影响。
影响因子：
4
作者：
R. P. McIntosh;J. McIntosh
通讯作者：
J. McIntosh
睾酮调节黄体生成素和卵泡刺激素的差异释放，这是对雄性猴（食蟹猴）促性腺激素释放激素脉冲频率变化的反应。
影响因子：
3.6
作者：
Adams,LA;Clifton,DK;Bremner,WJ;Steiner,RA
通讯作者：
Steiner,RA
垂体黄体生成素对男性静脉内和皮下注射促性腺激素释放激素的反应。
DOI：
10.1210/jcem-61-5-890
发表时间：
1985
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Spratt,DI;CrowleyJr,WF;Butler,JP;Hoffman,AR;Conn,PM;Badger,TM
通讯作者：
Badger,TM
垂体促性腺信号频率和恒河猴垂体-卵巢系统的功能。
影响因子：
4.8
作者：
Pohl,CR;Richardson,DW;Hutchison,JS;Germak,JA;Knobil,E
通讯作者：
Knobil,E