Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart.
Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart.
复制标题
DOI：
10.1242/dev.194563
复制
发表时间：
2021-02-03
期刊：
影响因子：
--
通讯作者：
Riley PR
中科院分区：
文献类型：
--
作者：
Cahill TJ;Sun X;Ravaud C;Villa Del Campo C;Klaourakis K;Lupu IE;Lord AM;Browne C;Jacobsen SEW;Greaves DR;Jackson DG;Cowley SA;James W;Choudhury RP;Vieira JM;Riley PR
巨噬细胞是先天免疫系统的组成部分，在组织炎症和修复中起关键作用。现在很明显，巨噬细胞也支持器官发生，但很少有研究表征其身份，个体发育和心脏发育过程中的功能。在这里，我们表明，驻地巨噬细胞的心外膜下室的发展心脏的分布和患病率与出现新的淋巴管，巨噬细胞密切相互作用与新生毛细淋巴管。因此，整体巨噬细胞缺乏导致广泛的血管破裂，突变的心脏表现出缩短和错误的模式化血管。心脏巨噬细胞的起源与卵黄囊和胎肝有关。此外，发现Cx3cr1+髓系在淋巴管内皮重塑中发挥重要作用。从机制上讲，巨噬细胞透明质酸是淋巴发芽所需的介导直接巨噬细胞淋巴内皮细胞的相互作用。总之，这些发现揭示了在哺乳动物心脏血管系统发育过程中巨噬细胞作为淋巴生长不可或缺的介质的作用。 组织中的巨噬细胞是心脏发育过程中淋巴管形成不可缺少的介质，并具有重塑血管丛的功能。
Macrophages are components of the innate immune system with key roles in tissue inflammation and repair. It is now evident that macrophages also support organogenesis, but few studies have characterized their identity, ontogeny and function during heart development. Here, we show that the distribution and prevalence of resident macrophages in the subepicardial compartment of the developing heart coincides with the emergence of new lymphatics, and that macrophages interact closely with the nascent lymphatic capillaries. Consequently, global macrophage deficiency led to extensive vessel disruption, with mutant hearts exhibiting shortened and mis-patterned lymphatics. The origin of cardiac macrophages was linked to the yolk sac and foetal liver. Moreover, the Cx3cr1+ myeloid lineage was found to play essential functions in the remodelling of the lymphatic endothelium. Mechanistically, macrophage hyaluronan was required for lymphatic sprouting by mediating direct macrophage-lymphatic endothelial cell interactions. Together, these findings reveal insight into the role of macrophages as indispensable mediators of lymphatic growth during the development of the mammalian cardiac vasculature. Tissue-resident macrophages are indispensable mediators of lymphatic vessel formation during heart development, and function to remodel the vascular plexus.
登录
查看更多内容
影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
DOI：
10.1084/jem.20072168
发表时间：
2008-05-12
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Benz C;Martins VC;Radtke F;Bleul CC
通讯作者：
Bleul CC
影响因子：
4.6
作者：
Chen, Heidi I.;Sharma, Bikram;Red-Horse, Kristy
通讯作者：
Red-Horse, Kristy
影响因子：
20.3
作者：
Fantin, Alessandro;Vieira, Joaquim M.;Ruhrberg, Christiana
通讯作者：
Ruhrberg, Christiana
影响因子：
20.3
作者：
Iqbal, Asif J.;McNeill, Eileen;Greaves, David R.
通讯作者：
Greaves, David R.