Localization of GABA, GAD65 and GAD67 in Rat Epiphyseal Growth Plate Chondrocytes
Localization of GABA, GAD65 and GAD67 in Rat Epiphyseal Growth Plate Chondrocytes
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GABA、GAD65 和 GAD67 在大鼠骨骺生长板软骨细胞中的定位
DOI：
10.1267/ahc.34.201
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发表时间：
2001
影响因子：
2.4
通讯作者：
Masahito Watanabe
中科院分区：
文献类型：
--
作者：
T. Tamayama;K. Kanbara;K. Maemura;M. Kuno;Masahito Watanabe
用免疫组织化学方法研究了γ-氨基丁酸（GABA）及其合成酶谷氨酸脱羧酶（GAD）在大鼠长骨骺生长板中的分布。GABA、GAD 65和GAD 67免疫反应阳性软骨细胞见于生长板的肥大区，而储备区和增殖区则无。在肥大区，GABA的免疫反应更强烈的成熟区比在变性区和临时钙化区。GAD 65和GAD 67的免疫反应模式几乎与GABA的免疫反应模式相当。采用逆转录-聚合酶链反应（RT-PCR）检测GAD 65和GAD 67 mRNA的表达。这些结果表明，GABA，这是从头合成的软骨细胞的生长板可能有一定的功能作用，在骨骼的生长。
The distribution of γ-aminobutyrate (GABA) and its synthesizing enzyme glutamate decarboxylase (GAD) in the epiphyseal growth plate of the rat long bone was studied by immunohistochemistry. GABA, GAD65 and GAD67 immunoreactive chondrocytes were found in hypertrophic zones of the growth plate, but not in reserve and proliferative zones. In the hypertrophic zone, GABA immunoreactivity was more intense in the maturation zone than in the degenerative zone and the zone of provisional calcification. Patterns of immunoreactivity for GAD65 and GAD67 were almost comparable to those of GABA. In addition, GAD65 and GAD67 mRNAs were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). These results suggest that GABA, which is synthesized de novo in chondrocytes of the growth plate may have certain functional roles in skeletal growth.
DOI：
10.1073/pnas.89.6.2115
发表时间：
1992-03-15
影响因子：
11.1
作者：
BU, DF;ERLANDER, MG;TOBIN, AJ
通讯作者：
TOBIN, AJ
生长板生理学和病理学。
DOI：
--
发表时间：
1990
期刊：
The Orthopedic clinics of North America
影响因子：
--
作者：
Iannotti,JP
通讯作者：
Iannotti,JP