Antibody inhibition of human cytomegalovirus spread in epithelial cell cultures.
Antibody inhibition of human cytomegalovirus spread in epithelial cell cultures.
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DOI：
10.1016/j.jviromet.2013.04.015
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发表时间：
2013-09
影响因子：
3.1
通讯作者：
McVoy MA
中科院分区：
文献类型：
--
作者：
Cui X;Lee R;Adler SP;McVoy MA
抗巨细胞病毒（CMV）抗体可降低CMV传播的发生率，并改善CMV相关疾病的严重程度。中和活性是抗体阻止无细胞病毒进入的能力，是天然免疫的重要组成部分。然而，体内CMV扩增可能主要通过组织内相邻细胞之间的扩散发生。因此，当评价治疗性抗体或对感染或免疫的体液应答时，细胞间扩散的抑制可能是重要的。体外CMV细胞间传播在很大程度上对成纤维细胞培养物中的抗体具有抗性，但在内皮细胞培养物中敏感。在本研究中，CMV超免疫球蛋白或血清阳性人血清中的抗体抑制上皮细胞培养物中CMV细胞间的传播。使用GFP标记的CMV株Towne或AD 169的亲上皮变体，用GFP报告基因测定定量扩散抑制活性。扩散抑制的测量为评价候选疫苗或免疫治疗剂提供了额外的参数，并进一步表征抗体在控制CMV传播和疾病中的作用。
Anti-cytomegalovirus (CMV) antibodies reduce the incidence of CMV transmission and ameliorate the severity of CMV-associated disease. Neutralizing activity, measured as the ability of antibodies to prevent entry of cell-free virus, is an important component of natural immunity. However, in vivo CMV amplification may occur mainly via spread between adjacent cells within tissues. Thus, inhibition of cell-to-cell spread may be important when evaluating therapeutic antibodies or humoral responses to infection or immunization. In vitro CMV cell-to-cell spread is largely resistant to antibodies in fibroblast cultures but sensitive in endothelial cell cultures. In the present study antibodies in CMV hyperimmuneglobulin or seropositive human sera inhibited CMV cell-to-cell spread in epithelial cell cultures. Spread inhibition activity was quantitated with a GFP reporter assay employing GFP-tagged epithelialtropic variants of CMV strains Towne or AD169. Measurement of spread inhibition provides an additional parameter for the evaluation of candidate vaccines or immunotherapeutics and to further characterize the role of antibodies in controlling CMV transmission and disease.
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影响因子：
6.4
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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