The impact of genome length on replication and genome stability of the herpesvirus guinea pig cytomegalovirus.
The impact of genome length on replication and genome stability of the herpesvirus guinea pig cytomegalovirus.
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DOI：
10.1016/j.virol.2008.12.030
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发表时间：
2009-03-30
期刊：
影响因子：
3.7
通讯作者：
McVoy, Michael A.
中科院分区：
文献类型：
--
作者：
Cui, Xiaohong;McGregor, Alistair;Schleiss, Mark R.;McVoy, Michael A.
评估了豚鼠巨细胞病毒（GPCMV）（疱疹病毒科成员）的基因组长度对复制和基因组稳定性的影响。 233 kb 的基因组可以减少 15.1 kb，而对病毒体外复制效率没有明显影响。基因组长度不足 31 kb 的病毒复制效率会降低，但这似乎是由于增强病毒基因的丢失而不是基因组长度的缩短所致。两个本身不致命的缺失在组合时却是致命的，这表明所得的 40.1 kb 长度不足的基因组低于最小可包装大小。基因组长度超过 8.8 kb，导致主要立即早期基因座右侧自发缺失，该区域在人类和恒河猴巨细胞病毒的成纤维细胞传代过程中经历缺失。这些结果表明，应该确认疱疹病毒突变体的基因组完整性，其中基因组长度即使适度增加。
The impact of genome length on replication and genome stability was assessed for guinea pig cytomegalovirus (GPCMV), a member of the Herpesviridae. The 233-kb genome could be decreased by 15.1 kb without discernable impact on viral replication efficiency in vitro. Viruses with genomes under-length by up to 31 kb replicated with decreased efficiencies but this appeared to arise from the loss of augmenting viral genes rather than decreased genome length. Two deletions that were non-lethal on their own were lethal when combined, suggesting that the resulting 40.1 kb under-length genome fell below a minimum packageable size. Genomes over-length by 8.8 kb gave rise to spontaneous deletions just to the right of the major immediate early locus, the same region that undergoes deletions during fibroblast passage of human and rhesus cytomegaloviruses. These results suggest that genome integrity should be confirmed for herpesvirus mutants in which genome length is increased even modestly.
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