Iron biofortification of myanmar rice.
Iron biofortification of myanmar rice.
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DOI：
10.3389/fpls.2013.00158
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发表时间：
2013
影响因子：
5.6
通讯作者：
Nishizawa NK
中科院分区：
文献类型：
--
作者：
Aung MS;Masuda H;Kobayashi T;Nakanishi H;Yamakawa T;Nishizawa NK
缺铁会增加人类的死亡率，特别是在发展中国家。在缅甸，儿童和孕妇缺铁性贫血的患病率分别为75%和71%。缅甸是全球人均大米消费率最高的国家之一。因此，生产铁生物强化大米可能有助于解决这一人群的铁缺乏问题。为了通过转基因方法生产铁生物强化缅甸大米，我们首先分析了15个品种的愈伤组织诱导和再生效率，这些品种目前因其高产或优质而受到欢迎。在每个品种的愈伤组织的形成和再生效率的强烈影响的类型的培养基含有一系列的2，4-二氯苯氧乙酸浓度。在愈伤组织诱导和再生试验中，具有高铁含量的Paw San Yin品种在抛光种子中表现良好。因此，我们转化了这个品种使用的基因表达盒，增强铁运输水稻植株内通过过表达的烟酰胺合酶基因HvNAS 1，铁流到胚乳通过Fe（II）-烟酰胺转运蛋白基因OsYSL 2，和Fe积累在胚乳中的Fe储存蛋白基因HvNAS 2。成功获得了具有转基因插入的品系。导入基因OsYSL 2、HvNAS 1和HvferH 2在未成熟T2种子中的表达增强。与非转基因水稻中的水平相比，转化体在T2抛光种子中积累了3.4倍高的Fe浓度，并且还积累了1.3倍高的锌浓度。这种铁生物强化大米有可能减少数百万缅甸人的缺铁性贫血，而不改变饮食习惯，也不增加额外成本。
Iron (Fe) deficiency elevates human mortality rates, especially in developing countries. In Myanmar, the prevalence of Fe-deficient anemia in children and pregnant women are 75 and 71%, respectively. Myanmar people have one of the highest per capita rice consumption rates globally. Consequently, production of Fe-biofortified rice would likely contribute to solving the Fe-deficiency problem in this human population. To produce Fe-biofortified Myanmar rice by transgenic methods, we first analyzed callus induction and regeneration efficiencies in 15 varieties that are presently popular because of their high-yields or high-qualities. Callus formation and regeneration efficiency in each variety was strongly influenced by types of culture media containing a range of 2,4-dichlorophenoxyacetic acid concentrations. The Paw San Yin variety, which has a high-Fe content in polished seeds, performed well in callus induction and regeneration trials. Thus, we transformed this variety using a gene expression cassette that enhanced Fe transport within rice plants through overexpression of the nicotianamine synthase gene HvNAS1, Fe flow to the endosperm through the Fe(II)-nicotianamine transporter gene OsYSL2, and Fe accumulation in endosperm by the Fe storage protein gene SoyferH2. A line with a transgene insertion was successfully obtained. Enhanced expressions of the introduced genes OsYSL2, HvNAS1, and SoyferH2 occurred in immature T2 seeds. The transformants accumulated 3.4-fold higher Fe concentrations, and also 1.3-fold higher zinc concentrations in T2 polished seeds compared to levels in non-transgenic rice. This Fe-biofortified rice has the potential to reduce Fe-deficiency anemia in millions of Myanmar people without changing food habits and without introducing additional costs.
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影响因子：
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作者：
Lucca, P;Hurrell, R;Potrykus, I
通讯作者：
Potrykus, I
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Nishizawa, Naoko K.
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4.6
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通讯作者：
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