Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition.
Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition.
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DOI：
10.1038/srep00543
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发表时间：
2012
影响因子：
4.6
通讯作者：
Nishizawa, Naoko K.
中科院分区：
文献类型：
--
作者：
Masuda, Hiroshi;Ishimaru, Yasuhiro;Aung, May Sann;Kobayashi, Takanori;Kakei, Yusuke;Takahashi, Michiko;Higuchi, Kyoko;Nakanishi, Hiromi;Nishizawa, Naoko K.
缺铁性贫血是全球最普遍的人类微量营养素缺乏症之一，为了解决缺铁性贫血问题，采用三种转基因方法生产铁生物强化稻米：通过使用胚乳特异性启动子表达铁储存蛋白铁蛋白来增强谷物中的铁储存量，通过天然金属螯合剂烟酰胺的过量产生来增强铁的易位，以及增加铁进入谷物的铁通量。 胚乳通过在胚乳特异性启动子和蔗糖转运蛋白启动子的控制下铁(II)-烟酰胺转运蛋白OsYSL2的表达来实现。我们的结果表明，温室种植的 T2 抛光种子中的铁浓度比非转基因种子高 6 倍，稻田生长的 T3 抛光种子中的铁浓度比非转基因种子高 4.4 倍，且产量没有缺陷。此外，转基因种子在田间积累的锌含量高达1.6倍。我们的结果表明，引入多个铁稳态基因对于铁生物强化比单个引入单个基因更有效。
To address the problem of iron-deficiency anemia, one of the most prevalent human micronutrient deficiencies globally, iron-biofortified rice was produced using three transgenic approaches: by enhancing iron storage in grains via expression of the iron storage protein ferritin using endosperm-specific promoters, enhancing iron translocation through overproduction of the natural metal chelator nicotianamine, and enhancing iron flux into the endosperm by means of iron(II)-nicotianamine transporter OsYSL2 expression under the control of an endosperm-specific promoter and sucrose transporter promoter. Our results indicate that the iron concentration in greenhouse-grown T2 polished seeds was sixfold higher and that in paddy field-grown T3 polished seeds was 4.4-fold higher than that in non-transgenic seeds, with no defect in yield. Moreover, the transgenic seeds accumulated zinc up to 1.6-times in the field. Our results demonstrate that introduction of multiple iron homeostasis genes is more effective for iron biofortification than the single introduction of individual genes.
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影响因子：
4.9
作者：
Kakei Y;Yamaguchi I;Kobayashi T;Takahashi M;Nakanishi H;Yamakawa T;Nishizawa NK
通讯作者：
Nishizawa NK
DOI：
10.1074/jbc.m110.180026
发表时间：
2011-02-18
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nozoye T;Nagasaka S;Kobayashi T;Takahashi M;Sato Y;Sato Y;Uozumi N;Nakanishi H;Nishizawa NK
通讯作者：
Nishizawa NK
影响因子：
30.8
作者：
KURATA, N;NAGAMURA, Y;MINOBE, Y
通讯作者：
MINOBE, Y
影响因子：
4.8
作者：
Masuda, T;Goto, F;Yoshihara, T
通讯作者：
Yoshihara, T
影响因子：
4.3
作者：
Kobayashi, T;Nakanishi, H;Mori, S
通讯作者：
Mori, S