A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae.
A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae.
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DOI：
10.1186/1741-7007-5-28
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发表时间：
2007-07-10
期刊：
影响因子：
5.4
通讯作者：
Kuroiwa T
中科院分区：
文献类型：
--
作者：
Nozaki H;Takano H;Misumi O;Terasawa K;Matsuzaki M;Maruyama S;Nishida K;Yagisawa F;Yoshida Y;Fujiwara T;Takio S;Tamura K;Chung SJ;Nakamura S;Kuroiwa H;Tanaka K;Sato N;Kuroiwa T
所有以前报道的真核生物核基因组序列都是不完整的，特别是在高度重复的单位和染色体末端。因为重复DNA对生物学的许多方面都很重要，所以完整的染色体结构是理解真核细胞的基础。我们早些时候几乎完整的温泉红藻蓝藻的基因组序列揭示了几个独特的特征，包括只有三个核糖体DNA拷贝，非常少的内含子，以及少量的基因总数。然而，由于某些功能上重要的重复元件的确切结构仍然不明确，该序列并不完整。显然，这些歧义需要解决，然后才能总结独特的特征，而这些歧义只能通过完成序列来解决。因此，我们的目标是完成之前的所有缺口并对所有剩余的染色体末端进行测序，现在报告第一个100%完成的真核生物的核基因组序列。我们目前的全序列由16546747个核苷酸组成，覆盖了从端粒到端粒的20条线性染色体的100%，代表了真核细胞简单而独特的染色体结构。我们已经明确地确定，美罗华丝虫基因组包含已知的最小的组蛋白基因簇，所有染色体末端都有一个独特的端粒重复，以及极少的转座子数量。根据这些属性和我们之前发现的其他属性，斑点锦鸡儿似乎拥有非共生真核生物中最简单的核基因组。这些在100%完整基因组序列中的异常简单的基因组特征对于进一步研究真核细胞非常有用。
All previously reported eukaryotic nuclear genome sequences have been incomplete, especially in highly repeated units and chromosomal ends. Because repetitive DNA is important for many aspects of biology, complete chromosomal structures are fundamental for understanding eukaryotic cells. Our earlier, nearly complete genome sequence of the hot-spring red alga Cyanidioschyzon merolae revealed several unique features, including just three ribosomal DNA copies, very few introns, and a small total number of genes. However, because the exact structures of certain functionally important repeated elements remained ambiguous, that sequence was not complete. Obviously, those ambiguities needed to be resolved before the unique features of the C. merolae genome could be summarized, and the ambiguities could only be resolved by completing the sequence. Therefore, we aimed to complete all previous gaps and sequence all remaining chromosomal ends, and now report the first nuclear-genome sequence for any eukaryote that is 100% complete. Our present complete sequence consists of 16546747 nucleotides covering 100% of the 20 linear chromosomes from telomere to telomere, representing the simple and unique chromosomal structures of the eukaryotic cell. We have unambiguously established that the C. merolae genome contains the smallest known histone-gene cluster, a unique telomeric repeat for all chromosomal ends, and an extremely low number of transposons. By virtue of these attributes and others that we had discovered previously, C. merolae appears to have the simplest nuclear genome of the non-symbiotic eukaryotes. These unusually simple genomic features in the 100% complete genome sequence of C. merolae are extremely useful for further studies of eukaryotic cells.
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影响因子：
12.3
作者：
Celniker SE;Wheeler DA;Kronmiller B;Carlson JW;Halpern A;Patel S;Adams M;Champe M;Dugan SP;Frise E;Hodgson A;George RA;Hoskins RA;Laverty T;Muzny DM;Nelson CR;Pacleb JM;Park S;Pfeiffer BD;Richards S;Sodergren EJ;Svirskas R;Tabor PE;Wan K;Stapleton M;Sutton GG;Venter C;Weinstock G;Scherer SE;Myers EW;Gibbs RA;Rubin GM
通讯作者：
Rubin GM
影响因子：
64.8
作者：
Matsuzaki, M;Misumi, O;Kuroiwa, T
通讯作者：
Kuroiwa, T
影响因子：
--
作者：
Cunningham, Francis X., Jr.;Lee, Hansel;Gantt, Elisabeth
通讯作者：
Gantt, Elisabeth
影响因子：
14.9
作者：
Christie, KR;Weng, S;Cherry, JM
通讯作者：
Cherry, JM
DOI：
10.1073/pnas.182432999
发表时间：
2002-09-17
影响因子：
11.1
作者：
Martin, W;Rujan, T;Penny, D
通讯作者：
Penny, D