Centriole age underlies asynchronous primary cilium growth in mammalian cells.
Centriole age underlies asynchronous primary cilium growth in mammalian cells.
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DOI：
10.1016/j.cub.2009.07.034
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发表时间：
2009-09-15
期刊：
影响因子：
--
通讯作者：
Stearns T
中科院分区：
文献类型：
--
作者：
Anderson CT;Stearns T
初级纤毛是基于微管的感觉细胞器，存在于大多数哺乳动物组织中，在发育和疾病中发挥重要作用。它们是Sonic hedgehog （Shh）和PDGF信号通路所必需的。初级纤毛由中心体的两个中心粒中较老的那个生长，称为母中心粒。在循环细胞中，纤毛通常在G1期生长，并在有丝分裂前消失，但对其生长的调控知之甚少。G1/S的中心粒复制产生两个中心体，一个具有较老的母中心粒，另一个具有新的母中心粒，它们在有丝分裂中分离。我们报道，由于有丝分裂，姐妹细胞中的初级纤毛是不同步生长的，接受母体中心粒的姐妹细胞通常先生长初级纤毛。我们还发现信号蛋白inversin和PDGFRα在姐妹细胞原纤毛上的定位是不对称的，姐妹细胞对Sonic hedgehog基因的反应是不对称的。这些结果表明，不同年龄的母细胞中心粒的分离，即每一种动物细胞分裂所固有的不对称性，可以影响姐妹细胞对环境信号的反应能力，可能改变一个或两个姐妹细胞的行为或命运。
Primary cilia are microtubule-based sensory organelles that are present in most mammalian tissues and play important roles in development and disease. They are required for the Sonic hedgehog (Shh) and PDGF signalling pathways. Primary cilia grow from the older of the two centrioles of the centrosome, referred to as the mother centriole. In cycling cells the cilium typically grows in G1 and is lost before mitosis, but the regulation of its growth is poorly understood. Centriole duplication at G1/S results in two centrosomes, one with an older mother centriole and one with a new mother centriole, that are segregated in mitosis. We report that primary cilia grow asynchronously in sister cells resulting from a mitotic division, and that the sister cell receiving the older mother centriole usually grows a primary cilium first. We also show that the signaling proteins inversin and PDGFRα localize asynchronously to sister cell primary cilia, and that sister cells respond asymmetrically to Sonic hedgehog. These results suggest that the segregation of differently aged mother centrioles, an asymmetry inherent to every animal cell division, can influence the ability of sister cells to respond to environmental signals, potentially altering the behavior or fate of one or both sister cells.
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影响因子：
56.9
作者：
Rohatgi, Rajat;Milenkovic, Ljiljana;Scott, Matthew P.
通讯作者：
Scott, Matthew P.
影响因子：
5.2
作者：
Heo, Jung Sun;Lee, Min Young;Han, Ho Jae
通讯作者：
Han, Ho Jae
影响因子：
21.3
作者：
Wong, C;Stearns, T
通讯作者：
Stearns, T
影响因子：
64.8
作者：
Corbit, KC;Aanstad, P;Reiter, JF
通讯作者：
Reiter, JF
DOI：
10.1073/pnas.78.8.4985
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARIANI, BD;SLATE, DL;SCHIMKE, RT
通讯作者：
SCHIMKE, RT