Differential expression of genes in HepG2 cells caused by UC001kfo RNAi as shown by RNA-seq
Differential expression of genes in HepG2 cells caused by UC001kfo RNAi as shown by RNA-seq
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RNA-seq显示UC001kfo RNAi引起的HepG2细胞中基因的差异表达
DOI：
10.1007/s11596-017-1765-1
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Pan YF
中科院分区：
文献类型：
--
作者：
Pan YF
利用RNA-seq技术研究UC 001 kfo RNAi对HepG 2细胞基因表达的影响。用Lenti-shUC 001 kfo慢病毒颗粒感染HepG 2细胞。实时荧光定量PCR检测UC 001 kfo mRNA在HepG 2-shUC 001 kfo细胞中的表达。RNA-seq技术用于鉴定HepG 2细胞系中由lncRNA UC 001 kfo调控的基因表达的差异。通过基因本体和信号通路分析，揭示了编码显著不同mRNA的基因的生物学功能。结果表明，HepG 2-shUC 001 kfo细胞株与HepG 2细胞株之间存在差异表达。UC 001 kfo mRNA在稳定细胞系HepG 2-shUC 001 kfo中表达显著下调（P<0.001）。此外，我们发现了19个信号通路或功能分类，包括30个在癌症特征、细胞粘附、侵袭和迁移中发挥作用的基因。结果还表明，随着lncRNA UC 001 kfo的下调，许多与癌细胞侵袭和转移相关的基因表达降低。LncRNA UC 001 kfo可能在调节癌细胞侵袭和转移中起作用。提示lncRNA-UC 001 kfo下调后，HepG 2细胞中mRNA的表达存在差异。部分参与细胞外基质、细胞粘附、运动、生长和定位。这些差异表达的基因可能参与了细胞的侵袭和转移。
The differential expression of genes in HepG2 cells caused by UC001kfo RNAi was investigated using RNA-seq. HepG2 cells were infected by Lenti-shUC001kfo lentivirus particles. The expression of UC001kfo mRNA in the HepG2-shUC001kfo cell line was detected by real-time PCR. RNA-seq technology was used to identify the difference in the expression of genes regulated by lncRNA UC001kfo in the HepG2 cell line. Gene ontology and signaling pathway analysis were performed to reveal the biological functions of the genes encoding of significantly different mRNAs. The results showed that mRNAs were differentially expressed between the HepG2-shUC001kfo cell line and the HepG2 cell line. The UC001kfo mRNA was significantly down-regulated in the stable cell line HepG2-shUC001kfo (P<0.001). Additionally, we found 19 signaling pathways or functional classifications encompassing 30 genes that played a role in cancer characteristics, cell adhesion, invasion and migration. The results also showed that the expression of many genes associated with cancer cell invasion and metastasis was decreased with the down-regulation of the lncRNA UC001kfo. LncRNA UC001kfo may play a role in regulating cancer cell invasion and metastasis. It was suggested that mRNAs were differentially expressed in the HepG2 cell line after the down-regulation of lncRNA-UC001kfo. Some took part in the extracellular matrix, cell adhesion, motility, growth, and localization. The genes encoding of differentially expressed mRNAs may participate in cell invasion and metastasis.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.3
作者：
Pradhan, Madhumita;Baumgarten, Sarah C.;Frasor, Jonna
通讯作者：
Frasor, Jonna
影响因子：
2.7
作者：
Wharton, KA;Zimmermann, G;Scott, MP
通讯作者：
Scott, MP
DOI：
10.1186/1756-9966-30-75
发表时间：
2011-08-12
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Wong AW;Paulson QX;Hong J;Stubbins RE;Poh K;Schrader E;Nunez NP
通讯作者：
Nunez NP
影响因子：
5.2
作者：
Masaru Katoh
通讯作者：
Masaru Katoh